| Objective: Detection ofα1-antitrypsin(AAT), transferrin(TF), PRO2619 in cholangiocarcinoma tissues and AAT, TF in peripheral blood of patients with cholangiocarcinoma in the expression of clearance, to further validate the application of the pre-group two-dimensional gel electrophoresis-mass spectrometry separation of serum proteins in patients with bile duct cancer research findings, discussion of these tumor-associated protein and the relationship between the occurrence and development of cholangiocarcinoma, with a view to looking for new and effective marker of tumor, improve the level of the early diagnosis of cholangiocarcinoma. Methods: SP immunohistochemical method assay 42 cases of cholangiocarcinoma and 19 cases of bile duct carcinoma paraffin specimens to expression the protein level of AAT, TF and PRO2619, the application of immune nephelometry method and 20 cases of cholangiocarcinoma in patients with 10 cases of bile duct stones in patients with preoperative serum levels of AAT and TF, and contrast AAT, TF with traditional tumor markers CEA, CA19-9 in the diagnosis of cholangiocarcinoma application.Results:1. AAT, TF, PRO2619 in the bile duct cholangiocarcinoma and normal tissues: AAT in the bile duct cholangiocarcinoma and normal tissues positive rates were 83.3% and 47.4%, the difference has statistical significance (P<0.05); TF in the bile duct cholangiocarcinoma and normal tissues positive rates were 47.6% and 57.9%, the difference was not significant (P>0.05); PRO2619 in the duct cholangiocarcinoma and normal tissues positive rates were 40.5% and 78.9%, the difference has statistical significance (P<0.05).2. AAT in cholangiocarcinoma and cholangiocarcinoma of the expression level of the relationship between clinicopathological features: AAT expression in cholangiocarcinoma levels had no statistically significant relationship between patient's sex, age, tumor site, lymph node metastasis(P<0.05), with cholangiocarcinoma differentiation, around the infiltration and UICC staging, the relationship between the statistical are significance (P<0.05).3. TF in cholangiocarcinoma and cholangiocarcinoma of the expression level of the relationship between clinicopathological features: TF expression in cholangiocarcinoma levels had no statistically significant relationship between patient's sex, age, tumor location, differentiation degree, lymph node metastasis, around the infiltration and UICC staging(P>0.05).4. PRO2619 in cholangiocarcinoma and cholangiocarcinoma of the expression level of the relationship between clinicopathological features: PRO2619 expression in cholangiocarcinoma had no statistically significant relationship between patient's sex, age, tumor site, lymph node metastasis, around the infiltration and UICC staging(P>0.05),there is statistical significance with cholangiocarcinoma of the relationship between the degree of differentiation(P<0.05).5. AAT, TF, PRO2619 expression in cholangiocarcinoma relevance: AAT and TF expression in the cholangiocarcinoma was no correlation(r=0.17, P>0.05),AAT expression with no correlation with PRO2619(r=0.24, P>0.05),TF expression and no correlation with PRO2619(r=-0.01, P>0.05).6. AAT, TF measured in the peripheral blood-money value: the value of AAT in serum of patients with cholangiocarcinoma are 2.60±0.53g/L, serum of patients with bile duct stones in the value of AAT are 1.71±0.35g/L,there were significant differences between the two(P<0.05); the value of TF in serum of patients with cholangiocarcinoma are 1.98±0.48g/L, serum of patients with bile duct stones in the value of TF are 2.46±0.23g/L, there were significant differences between the two(P<0.05).7. Compared the rate of abnormal about AAT, TF values and CA19-9, CEA values in cholangiocarcinoma serum: cholangiocarcinoma in patients with AAT, TF, CA19-9, CEA values were abnormal rate of 80%, 60%, 75%, 45%. AAT value of the abnormal rate compared with CA19-9, the difference is not statistically significant(P>0.05); the abnormal rate of AAT value compared with the CEA,the abnormal, the difference has statistical significance(P <0.05); the abnormal rate of TF value compared with CA19-9, CEA, there were no statistical significance(P>0.05).Conclusions:1. AAT expression in cholangiocarcinoma was significantly higher than normal degree of bile duct organization, but with cholangiocarcinoma of the lower degree of differentiation, invasion of surrounding tissue and increase in stages UICC expression, AAT may be associated with cholangiocarcinoma prompted vicious close evolutionary process.2. TF in cholangiocarcinoma tissue and normal tissue expression of the degree of bile duct was no significant difference, and with the patient's sex, age, tumor location, differentiation degree, lymph node metastasis, UICC staging around the infiltration and the relationship between the non-relevance.3. PRO2619 at the level of bile duct carcinoma tissues was significantly higher than that of normal bile duct organization, but differentiation of bile duct cancer with lower levels of expression, PRO2619 probably prompted changes in the vicious and the degree of bile duct cancer.4. AAT clearance at peripheral blood of patients with cholangiocarcinoma was significantly higher than that measured in patients with bile duct stones, the abnormal rate of CA19-9 was no significant difference compared with the CEA has statistically significant difference, AAT can be used as a prompt and CA19-9 is similar to the role of tumor markers to monitor the occurrence of cholangiocarcinoma.5. TF in the peripheral blood of patients with cholangiocarcinoma was significantly lower than that measured in patients with bile duct stones, but the abnormal rate and the rate of CA19-9 abnormality, CEA abnormality rates were not statistically significance, suggesting that TF in the peripheral blood may be accompanied by the decrease in the occurrence and development of cholangiocarcinoma.
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