Study On The Serum Phospholipid Fatty Acid Profile In Obese Children And Adolescents

Wth the development of the social economy and the transconformation of people's lifestyle,obesity has become a global health problem.Furthermore,the prevalence of overweight and obesity has increased markedly in children and adolescents in the world over the last four decades,which has become a major health problem.Obesity in child-hood and adolescence is associated with adult obesity and the development of the metabolic syndrome.Insulin resistance,glucose intolerance and hyperinsulinaemia,hyperlipidemia, hepar adiposum which are major components of the metabolic syndrome,are highly prevalent among children and adolescents with marked obesity,which increased the risk of the prevalence of cardiovascular disease.Although obesity relative with gene,it can be induced by different environmental factors,including dietary habits. From a biochemical standpoint,different types of dietary fatty acids may play a critical role in adipocyte differentiation.The quality of dietary fat and not just its quantity is more related to risk of obesity.Epi-demiological evidence and intervention studies found that the fatty acid composition of one's usual diet was reflected in the composition of the fatty acid pattern in serum phospholipids.It was found that the degree of obesity are positively related with saturated fatty acid(SFA)and negatively related with polyunsaturated fatty acid(PUFA).The relationship between the serum phospholipid fatty acid profile and adiposity has been scantly investigated in younger ages, and results are controversial.Object:Studies comparing the the serum phospholipid fatty acid profile in obese children and adolescents to normal healthy group and analyze the bivariate correlation of clinical characteristics and serum phospholipid fatty acids in obesity group,discuss the association between serum phospholipid fatty acids and obesity and suggest suitable dietary intervention.Methods:41 obese children and adolescents and 40 healthy subjects were studied.There is no significant difference in age and gender between obesity and normal control group. Serum phospholipid fatty acid was analyzed with capillary gas chromatography.Blood biochemical parameters were test by the standard methods.The degree of fat liver were measured by color abdominal ultraonography.The data are processed by SPSS 13.0 for statistical analysis,with P＜0.05 considered statistically different.Results:1.Serum phospholipid SFA,C16:0,C16:1,C18:0,C18:3 n-6,C18:3 n-3, C20:0,C20:2n-6,C20:3n-6,C22:1,C16:1/C16:0,C20:3n-6/C18: 2n-6 in obesity group(percentage of total fatty acid)were significantly higher than those of normal control group,but PUFA,n-6PUFA,PUFA/SFA,C14:0,C18:1 n-7,C18:2 n-6,C20:1,C20:4n-6,C20:4n-6/C20:3n-6 were significantly lower than those of normal control group.2.Bivariate correlate showed ALT,AST was negatively correlated with C14:1 and positively correlated with C20:3n-6;TG was negatively correlated with C14:1,C20: 0,TC was negatively correlated with C14:1,C18:1n-7 and positively correlated with C20:3n-6,C22:4n-6,C22:5n-6,C24:0;HOMA-IR was positively correlated with C16:0,C18:0 and negatively correlated with C24:0.A11 the P value are＜0.05.3.Logistic stepwise regression analysis showed:WC,BMI,ALT,AST,FINS, HOMA-IR,C16:0,C18:0,C20:3 n-6,SFA was positively correlated with NAFLD,C18:1n-7,C18:2n-6,PUFA was negatively correlated with NAFLD.Conclusions:1.Serum phospholipid fatty acid profile was significantly different between childhood and adolescence obesity group and normal control group.SFA was significantly higher than those of normal control group,but PUFA,n-6PUFA were significantly lower than those of normal control group.2.Serum phospholipid fatty acid profile in childhood and adolescence obesity is significantly correlated with hyperlipidemia,Insulin resistance and hepar adiposum. People should emphasis the quality not just quantity of dietary fatty acid and increase the dietary levels of PUFA especially n-3PUFA and reduce the dietary levels of SFA,which may prevent the development of obesity and diseases correlation with obesity.