The Expression Of IFN-γ And Caspase-3 In The Gastric Tissues Of Gastric Ulcer Rats Through Acupuntcure Treatment

ObjectiveBy observing the acupuncture treatment of experimental gastric ulcer in rats,the changes of the area of the ulcer, the content of HP and the caspase-3,IFN-γexpression in the gastric tissues.Analysed the relationship between acupuncture therapy in gastric tissues and the content of caspase-3 and IFN-γto probe the mechanism of needling ST36(Zusanli) in the promotion of the healing and prevention of the recurrence of gastric ulcer.Methods90 SD rats of Clean-level,150-200 grams.All female. Preparation of gastric ulcer model by using acetic acid.After operation,in 3,8,14 days,rats were killed by Cervical dislocation method.Then cut open the abdominal cavity,removed the stomach,cut the stomach along the greater curvature. Placed the tissues on ice_Plate,washed them by Ice-cold saline. After measured the ulcer index(UI),the surrounding tissue of the ulcer were fixed by 10%formaldehyde solution.The tissue were preserved in ultra-low temperature refrigerator for the next test.The tissue were staind though HE and immunohistochemical.Measure the content of caspase-3 and IFN-γin rat gastric tissues.Used computer image analysis system to analyse.The result did statistical analysis. Results1.Visual observation:In the rats' acetic acid ulcer model, killed rats in the three days after the operation.The percent of ulcers on the mucosal of the anterior wall was 100%.The performance were typical gastric ulcer.The normal rats' gastric mucosal fold Obviously,light red,no congestion and edema,no ulcer.2.The change of the caspase-3's content in ulcer rat gastric tissue:The content of the caspase-3 in the blank group was 0.127±0.021,which in the model group was 0.263±0.033,in the electro-acupuncture group was 0.222±0.027.The caspase-3's levels of model group and electro-acupuncture group were significantly higher(P＜0.01)than the blank group's.Compared with model group,the content of caspase-3 of EA group were significantly lower(P＜0.05).3.The change of the IFN-γ's content in ulcer rat gastric tissue:The content of the IFN-γin the blank group was 0.321±0.022,which in the model group is 0.363±0.046,in the electro-acupuncture group was 0.323±0.021.The IFN-γ's levels of model group and electro-acupuncture group were significantly higher(P＜0.05) than the blank group's.Compared with model group,the content of IFN-γof EA group were significantly lower(P＜0.05).ConclusionElectro-acupuncture may reduce the UI and promote the recovery of gastric ulcer.Electro-acupuncture reduces the expression of caspase-3 and IFN-γin the rats' gastric tissue.The results Suggest that acupuncture is effective in the treatment of gastric ulcer and its mechanism might be relative to its effectiveness in reducing the content of caspase-3 and IFN-γin gastric tissues.Thereby acupuncture can block apoptosis.The reason why acupuncture can treat gastric ulcer may be associated with the effect that acupuncture can...