Experemental Study On The Effect Of Whole Peptidoglycan Of Bifidobacterium Inducing Lovo Cell Apoposis In Vitro

Objective:To explore the Effect of Whole Peptidoglycan of Bifid Bacterium Inducing Lovo Cell Apoptosis in vitro and its mechanisms.Methods:Human colorectal carcinoma cell line Lovo proliferation was analyzed by MTT after treated with WPG(10μg/ml,20μg/ml,40μg/ml,80μg/ml, 160μg/ml,320μg/ml,200ul/well)for 24h,48h and 72h.Human colorectal carcinoma cell line Lovo apoptosis was observed by Annexin-V-FITC/PI (Flow cytometry)after treated with WPG at concentrations on 80μg/ml for 72 h.The expression levels of NF-κB of human colorectal carcinoma cell line Lovo were assessed by immunocytochemistry after treated with WPG at concentrations on 80μg/ml for 72h.Results:Methyl thiazolyl tetrazolium(MTT)assay show that WPG at concentrations on 10-320μg/ml produced cytotoxic effect on the cell in vitro and could obviously inhibit proliferation of human colorectal carcinoma cell line Lovo with dose-dependent relation and time-dependent relation in vitro.Detected by Annexin-V/PI double labeling flow-cytometry,the apoptosis rate of human colorectal carcinoma cell line Lovo treated with 80μg/ml for 72 h was 36.75%,higher than that in the untreatment human colorectal carcinoma cell line Lovo wich was 12.02%(P＜0.01).The immunocytochemistry analysis showed that the levels of NF-kB expression from human colorectal carcinoma cell line Lovo treated with 80μ/ml for 72 h was significantly lower than that in the untreatment human colorectal carcinoma cell line Lovo(p＜0.01).Conclusion:LMAPAB can inhibit the proliferation of human colorectal carcinoma cell line Lovo significantly and its mechanism is interrelated to inhibiting the activation and the expression level of nuclear factor kappa B and thus inducing the early apoptosis.