Study On Function With Schedul Hot And Dehygrosis Method To The Bile Siltation Liver Fibrosis Big Mouse And The Liver Membrane Transportation Body Mrp2 Change Rule

Cholestasis is by many kinds of reason result choleresis or the excretion barrier, causes the bile not to be able to flow in the duodenum normally, thus the regurgitation enters in the blood. On clinical often the performance is the jaundice, the pruritus, urinates the color to be deep, the excrement color shoal or the xanthelasma and so on, the laboratory inspection may have TBil, ALP, 5- nucleotidases and GGT the level elevates, ALT and AST prompts has the liver cell damage, the chronic bile siltation common total cholesterol level elevates.Objective:The observation has the refrigeration to dispel the wet effect three traditional Chinese medicine compound prescription Herbae Artemisiae Capillariae Decoction, Artenisiae Scopariae and Poriae Powder and tdaisaikoto to the bile siltation liver fibrosis big mouse's pharmacological action and the liver membrane transportation body Mrp2 change rule research.Method:Uses outside the liver the bile duct ligation (BDL) and Alpha-naphthylisothiocyanate (ANIT) fills the stomach method to induce the big mouse liver damage, forms the bile siltation liver fibrosis model, by the Herbae Artemisiae Capillariae Decoction, Artenisiae Scopariae and Poriae Powder and tdaisaikoto carries on fills the stomach treatment, after making the mold separately takes four phase (the BDL model separately after making mold the 3rd day, the 1th week, the 2nd week, the 3rd week /ANIT model making mold the 24,48,72,96th hour) dynamic observation experimental nature big mouse ordinary circumstances, hepatorenal function change, liver organization pathology change and fibrosis degree as well as three formula medicinal preparation intervention function.Result:1.Compares with the sham-operation group, the BDL model group big mouse's body weight has varying degree reduction; Liver's weight, spleen's weight, the liver index, the spleen index assumes the markup the dynamic change; Through after making the mold the 3rd day, the 1st week, the 2nd week, the 3rd week-long big mouse liver HE dyeing pathology shape observation and the sirius red gallbladder dyes to the liver collagenous fibre observation, ties up 3 days later, collects the district to have the bile duct proliferation, to 3 weeks when the proliferation aggravates gradually; The blood serum Alb content drops continually; But GLO, TP content first elevation then reduction ; The TBil content, the GGT and ALP activeness assumes advances continually; The ALT activeness, the TBA and CHE content namely the remarkable markup makes the mold 3 days later, hereafter drops, to 3 weekend was still higher than the sham-operation group (P<0.01,P<0.05); AST 3 days later namely obviously advances and maintains to 3 weekends. The LDH activeness when makes the mold 1 week remarkable markup, after namely, drops; The RUN content after making the mold previous 2 weeks change not obviously, have the markup to the 3rd week; CRE content non-statistics significance.2.In the BDL model, compares with the model group, all medication intervention group body weight, liver's weight, liver/body weight ratio have reduce, but statistics is insignificant, spleen's weight, the spleen/body weight ratio obviously reduce(P<0.05); In three medication intervention group bile duct type epithelial cell or the small bile duct obvious reduction, the liver cell changes many relatively, the collagenous fibre proliferation reduces, the liver organization fibrosis degree reduces, is especially obvious by theHerbae Artemisiae Capillariae Decoction group curative effect; The Herbae Artemisiae Capillariae Decoction group ALB content, the LDH activeness increase; Various medicines intervention group TBil, the TBA content and the ALP activeness reduce; The Herbae Artemisiae Capillariae Decoction group and the Artenisiae Scopariae and Poriae Powder group TBA content, the GGT activeness obviously to reduce(P<0.01).3.Compares with the normal group, the ANIT model group big mouse's body weight has varying degree reduction, liver's weight, the liver index assumes the markup the dynamic change, spleen's weight, the spleen index advances first, the dynamic change which then when makes mold 72h reduces; After making the mold, 24h collects the district to have the bile duct epithelial cell proliferation, few inflammatory cell infiltration, when 72h the bile duct epithelial cell proliferation scope increases, the collagenous fibre proliferation, the 96h proliferation's small bile duct type epithelial cell starts to reduce; The Alb content drops, 96h close normal level; GLO, TP, the CHE content changes not obviously; TBil, TBA content, the ALT and AST active 24h and 72h obvious markup (P<0.05), 48h and 96h close normal; ALP, GGT remarkable markup P<0.05), when 96h close normal; The LDH activeness remarkable drop (P< 0.05), 96h elevates; When RUN content 24h drops, after namely, advances the close normal level; CRE content when 48h,72h advances (P<0.05), when 96h restores normally; The liver organizes the Mrp2 transportation protein the Mrp2 masculine gender expression quantity which organizes in the normal group liver to be most, in model group 24h, 48h, 72h Mrp2 masculine expression quantity reduces gradually, increases in the model group 96 hour Mrp2 masculine expression quantity.4.In the ANIT model, all medication intervention group body weight, liver's weight obviously reduces, Herbae Artemisiae Capillariae Decoction, daisaikoto's liver/body weight ratio obviously reduces (P<0.05), the artemisia capillaris five water chestnuts disperse liver/body weight ratio, all medication intervention group spleen heavy also has reduces, but non-statistics significance; Compares with the model group, three medication intervention group bile duct type epithelial cell or the small bile duct obvious reduction, the liver cell changes many relatively, the collagenous fibre proliferation reduces, the liver organization fibrosis degree reduces, is especially obvious by the artemisia capillaris soup group curative effect; The liver organizes the Mrp2 transportation protein to increase in three medication intervention group, particularly the artemisia capillaris soup is most obvious (P<0.05). Conclusion:1.Regardless of being the BDL model or the ANIT model, the bile siltation big mouse liver fibrosis model main pathology change has the bile to silt up, the bile duct epithelial cell proliferation, the liver cell to reduce, the collagen proliferation and the deposition, is close with the newborn bile duct epithelial cell relations .2.Further confirmed the bile siltation disease's pathogenesis which in the theory establishes bears the medicine related protein with the multi-medicines 2 (Mrp2) the expression related.3.Has the refrigeration to dispel the wet effectHerbae Artemisiae Capillariae Decoction, Artenisiae Scopariae and Poriae Powder, daisaikoto has the more remarkable anti-big mouse experimental liver fibrosis function, and is best by Artemisiae Capillariae Decoction, Artenisiae Scopariae treatment result, comes from the test result by the formula to examine the card, bile siltation disease by Hot Preponderancy Wet card primarily.