| RA is a kind of invasive chronic progressing autoimmune disease with arthrosynovitis and Destructive arthropathy as its main character, having long pathogenesis and recurrent attacks and high rate of mutilation and malformation. Though the fundamental factors of the occurrence and development of RA were known to be the activation of many kinds of immunocompetent cells, the cooperation of cytokine and phlogistic mediator and the formation of pannus,the extensional etiopathogenesis of RA is still unclear and is in need of further research. Untill recently the main method of RA theopy is to improve the symptom with the help of antirheumatic medication. But the traditional medications have strong adverse reaction and the prices of biologic agent were very high,leading to the compliance of patients and the difficulty of disease control. Therefore the emergence of a kind of antirheumatic medication with better potency ratio becomes more and more necessary and the crucial part of developing new effective medication and formulating therapeutic measure lays in the investigation and development of effective and reasonable RA model.This research did experiments on Wistar rats by immunizing the animals with different experimental agents and then evaluated the arthrositis index, serology, pathology, imageology and economics of the model synthetically. The methods were as follow: divided the 4-6 week-old's male Wistar rats into fine groups in random, group Ml (immunized with CFA by intradermal injection in voix pedis), group M2(immunized with chicken CII+IFA by intradermal injection in three spots of voix pedis, tail radix and back), group M3(immunized with chicken CII+IFA and treated ice water for 7 days), group M4(immunized with chicken CII) and the control group(without any treatment). The same experiment was performed after 7days to enhance the immunity in order to induce the model of RA. The thickness of rats' voix pedis were measured and the arthrositis indexes were evaluated at the 7d, 14d, 21d, 42d after the first immunity. Double antibody ELISA method was used to detected the level of anti-CII antibody, TNF-α, IL-1β, IL-17 at the 21d and 42d, and the changes of histopathology and radiology were also observed in order to evaluate the reliability of model- establishing method in this experiment.Results indicate that the voix pedis of the rats in group M1-M3 were thicker than that of group M4 and the control group (P<0.05) within 10 days after the first immunity, whereas the contradiction between group M4 and the control group had no statistical meanings. The level of blood serum anti-CII antibody of group M2 and M3 had a significant rise at the peak period of inflammation(P<0.01), while no remarkable change of anti-CII had been detected in group Ml and M4. The level of anti-CII, TNF-α, IL-1β, IL-17 in group M1-M3 was higher than that of the control group during the peak period(P<0.05), those inflammatory values declined afterward and were still above the normal level 42 days after the immunity. Pathologic histological section indicated the infiltration of plenty of inflammatory cells, hyperplasia of osteocyte, distruction of cartilage and bones and the formation of pannus within the synovium of joint of the rats in group M1-M3. X ray pictures showed severe swelling of parenchyma around ankle joint, and the gaps of the ankle joint and voix pedis joint became indefinite and stenotic with sclerotin destroyed as well.This research revealed that the rats immunized with CII and CFA and treated with ice water have great similarity with human RA in aspect of both appearance and histopathology, radiology evaluation, therefore this group proves to be more coincident with human RA compared with other groups. This finding provides valuable experimental information for further research into the mechanism of human RA and the development of new antirheumatic drugs. |
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