Clinical Significalnce Of HA117 Expression In Acute Leukemic Of Children

Objective To explore the correlation between the expression of HA117 gene in bone marrow mononuclear cells and its relationship with multidrug resistance.Methods HA117 gene expression in 36 acute leukemia patients, and 10 ITP patients controls were studied using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique.Results①The positive expression rate of HA117 gene in the patients was 75.00%. There was no significant difference in HA117 gene expression between ALL cells (69.57%) and AML cells (91.67%), but HA117/β-actin Semi-quantitative expression levels in AML were higher than that in ALL(q=4.5852,P<0.01).②HA117 gene expression and HA117/β-actin Semi-quantitative expression levels in ALL and AML were higher than that in ITP patients (χ~2 =5.05,8.81;q=4.4612,6.9695;P<0.05).③HA117 gene expression in initially treated patients were higher than that in ITP patients(χ~2=5.91,P<0.05),there was no significant difference form remission patients (χ~2=0.01,P>0.05),but HA117/β-actin Semi-quantitative expression levels in initially treated patients higher than remission patients and ITP patients(q=5.1991,6.3812,P<0.01).④HA117 gene expression in remission patients were higher than that in ITP patients(χ~2=4.24,P<0.05), but there was no significant difference in HA117/β-actin Semi-quantitative expression levels from ITP patients(q=1.6607,P>0.05).⑤HA117/β-actin Semi-quantitative expression levels in non- remission patients were higher than that in remission patients and ITP patients (q=3.1705,4.4102,P<0.05), but there was no significant difference from initially treated patients(q=0.5470,P>0.05).Conclusion①New gene HA117 was high expression in initially treated and non- remission patients in the BMMNC of children with AL, semi-quantitative expression of HA117 in remission patients was no difference between patients with ITP.②The experiments show that clinical testing of its quantitative expression levels was more meaningful.③HA117 gene level changed, Non-remission and AML group were high expression, ALL and remission group were low expression. HA117 was one of the factors which may affect the clinical remission rate.④Conclusions in light of the early experimental results combined with the experimental results, in the BMMNC of initially treated and non- remission patients, the leukemia cells proliferation were rapidly, while HA117 gene in the two groups was high expression; In remission group, leukemia cells proliferation was controlled, while HA117 gene was low expression. HA117 new gene expressed in tumor tissue, it may be involved in multidrug resistance of leukemia. HA117 may be one of the reasons which AL cells was not sensitive to chemotherapeutic drugs.