Expression Of Multidrug Resistance (MDR1) And Multidrug Resistance-associated Protein (MRP) Genes In Bladder Carcinomas And Normal Bladder

Objective: To study the expression levels of the multidrug resistance (MDR1)and multidrug resistance-associated protein (MRP )genes in bladder cell carcinoma and normal bladder, and to investigate the clinical singificance of both genes. Materials and Methods: Using a sensitive semiquantitative reverse transcripion-polymerase chain reaction(RT-PCR) assay we examined 13 bladders carninomas and 10 normal bladders for expression of both MDRl and MRP genes.The (32-MG gene was use as the internal control. Result: ?A11 samples examined were found to express the MDRl and MRP genes. MDRl in normal bladder and bladder carcinoma were0.69 ±0.21,0.46 +0.31,respectively,and MRP were 0.42 + 0.31,0.78 + 0.43 respectively. Comparisons of the expression levels between normal bladders and bladder carcinoma expression level was very lower in bladder cell carcinoma than in normal bladders (p <0.01) , but the mean MRP gene expression level was higher in bladder carcinomas (p<0.05). (2)No association was found between the expression level of MDRl and UICC stage, histological type or histological stage. So did MRP. (3)The expression levels of both MDRland MRP genes did not related to progression -free survival.? Significant correlation was found between the expression of MDRl and MRP genes in normal bladder (r=0.932.p<0.01) ,but not in bladder carcinoma. Conclusion: MDRl and MRP genes expressed in both normal bladder and bladder carcinoma, and related to intrisic multidrug resistance in bladder carcinoma. Measurement of the expression of MDRl and MRP genes in patients' tumor might help in selecting sensitive drugs to overcome drug resistance, therefore improve treating effects.