Study Of NS398 Reversing The Antergy Of Lysophospatidic Acid For The Inhibiting Effect Of Cisplatin In The Proliferation Of Huanman Ovarian Cancer

Objective:1. To detect the expression changes of cyclooxygenase-2(COX-2) induced by LPA on human ovarian cancer cell line (3AO).2. To investigate the effect of NS398 (COX-2 inhibitor) on the antergy of lysophosphatidic acid (LPA) for the inhibiting effect of cisplatin(DDP) in the proliferation of human ovarian cancer cells, and analyzing its possible mechanism.Methods:1.3AO were cultured in vitro. The effects of LPA or NS398 alone and LPA in combination with NS398 on the expression of COX-2 of 3AO cells were measured with RT-PCR and Western blot.2. 3AO were cultured in vitro, and set up different groups. There were DDP, LPA, NS398, LPA+DDP, NS398+LPA+DDP and control group. The cells of proliferation each group were measured by MTT. Cell cycle of 3AO were analysed by flow cytometry (FCM). Results:1. The results detected by RT-PCR and Western blot revealed that, after 12 hour 3AO cells was treated with 40μmol/L LPA, the expression of COX-2 in 3AO cells was increased,but in combining with NS398, the expression of COX-2 was decreased.2. The results analyzed by MTT and FCM, LPA show antergy on the the inhibiting effect of DDP in the proliferation of 3AO cells, and decrease the proportion of cells in the G0/G1 phase of the cell cycle. Whereas, combining NS398 in culture, the proliferation of cells were significantly inhibited, and the proportion of cells in the G0/G1 phase of the cell cycle was rised.Conclusions:The reverse effect of NS398 for the antergy of LPA on the the inhibiting effect of DDP in the proliferation of 3AO cells may be completed through inhibiting the expression of COX-2 in these cells.