| 0bjective: To investigate toxic effect of the cadmium(Cd) on the expression of associated proteins and the ultrastructure of Sertoli cell(Sc) in rat and antagonistic effect of Astragalus mongholicus(A) to it.Methods:①21 SD male rats, mean weight was 180.7±2.2g ,which were randomly divided into three group. Cd group ( SD rats of different interval from 1W to 4W times were treated intraperitoneally injected with 0.1% CdC12,1 mg/kg body weight daily, 5 days a week); Cd+A group (SD rats of different interval from 2W to 4W times were treated intraperitoneally injected with Cd C12, same as above for the dosage,and simultaneously injected with A 10g/Kg body weight daily, 5 days a week); Control group were treated intraperitoneally injected with equal dosage of normal saline. The testes treated by Cd, Cd+A and C respectively were studied by light, electron microscope and immunohistochemistry of vimentin(V) & E-cadherin(E-cad) and were semi-quanitatively analysed by image analysis. In addition, 6 SD male rats were randomly divided into Cd 1W group and C group. The testes treated by lanthanum nitrate(La) were studied by electron microscope.②Sc of primary culture about 95% purity were obtained from 16 days old SD male rats'testes. Then, Sc of primary culture treated with Cd, Cd+A and C were used for ultrastructureal observation, immunohistochemistry of V and E-cad and determination of calcium ion concentration.Results:①Sc of the irregula nucleus and obvious nucleolus were observed in H.E stain by light microscope. The vacuolated cytoplasm were observed on Sc treated by Cd. Never obvious changes were observed on Sc treated by Cd+A. Immunohistochemistry analysis in vivo demonstrated that the positive product of V was expressed in cytoplasm of Sc , which was radiate elongation to lumens in seminiferous tubules. The average optical density(AOD)of V was decreased obviously with the time extending treated by Cd. While the expression of positive product in cytoplasm was similar in Cd+A compared with C. Positive product of E-cad was localizated in cytoplasm of Sc and spermatogenic cell. AOD of E-cad treated with Cd as well as Cd+A were similar to expression of V. The electron microscope observation showed that the main ultrastructure of Sc included irregula nucleus and obvious nucleolus, after Cd treatment, the ultrastructure damage of organelles, BTB and Es in Sc were observed, and the destroyed degree were demonstrated time-dose dependent manner. Meanwhile. The tracing with La showed that TJ of Sc were infiltrated by La. The ultrastructure of organelles, BTB and Es of Sc were destroyed lightly in Cd+A.②Sc of primary culture showed the main ultrastructural character of Sc, including irregula nucleus, obvious nucleolus and bipolar corpuscula around nucleolus. Positive products of V and E-cad, which form reticular formation in Sc were lower in Cd group compared with C group (P<0.05),which in Cd+A group were lower than C group but higher than Cd group(P<0.05).The concentration of calcium ion in Sc were 81.773±3.711(C), 161.602±1.978(Cd), 104.278±1.963(C+A), respectively. Ultrastructure damage including cytoplasm vacuolated,heterochromatin aggregated or karyorrhexis were significantly found in Cd group,whereas slightly in Cd+A group.Conclusion: Our experiments demonstrated that Cd has obvious toxic effect on Sc both in vivo and in vitro.Cd decreased expression of V and E-cad, increased concentration of intracellular calcium ion in Sc and damaged BTB of Sc. A can against damage of Cd on Sc in rat.
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