| Objective To develop symptomatic cerebral vasospasm model following subarachnoid hemorrhage in rabbits by twice injecting nonheparinized autologus arterial blood into the cistern magana combined with ligation of the single side common cervical artery.Methods A total of 24 white rabbits ( New Zealand ) were divided randomly into 2 groups, including SAH group (twice injecting nonheparinized autologus arterial blood into the cistern magana), and S-SAH group (twice injecting nonheparinized autologus arterial blood into the cistern magana combined with ligation of the single side common cervical artery, n=10 ). The 2 rabbits were used for observing blood distribution of subarachnoid hemorrhage, and 2 were used as reserves. The neurological functional changes were observed before and after SAH. DSA was performed at the pre-operation, post-operation ( d1, d5 ) respectively in SAH and S-SAH groups. The basilar artery of SAH and S-SAH was detected by histological study after completing DSA.Results The rabbits in SAH and S-SAH group developed ratio of neurological disorder 30% and 70% respectively. Compared with rabbits in SAH group, the neurological disorder of rabbits in S-SAH group were more severe and lasted much longer; The existence of cerebral vasospasm was confirmed by DSA in SAH group and S-SAH group on d5( P<0.05 ); Both groups have no significant deviation in the degree of CVS ( P>0.05 ). There were pathological change in the basilar artery of SAH and S-SAH by histology on d5.Conclusions The animal model of cerebral vasospasm can be successfully developed in the rabbits by twice injecting nonheparinized autologus arterial blood into the cistern magana combined with ligation of the single side common cervical artery, which is economical and reproducible. It can elicit stable-symptomatic cerebral vasospasm following subarachnoid hemorrhage in rabbit and be used to investigate the mechanism of neurological disorder after SAH and efficacy of medical treatment.
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