Expression And Clinical Significance Of EGFR And EGFR Mutations In Primary Hepatocellular Carcinoma

Objective:To detect the expression of EGFR and EGFR mutations (EGFR mutations(del) and EGFR mutations(p)) in hepatocellular carcinoma tissue,explore their relationship with clinical pathology and provide theoretical basis and clinical guideline for the molecular target treatment of hepatocellular carcinoma.Methods:78 cases of fresh specimens were resected in hepatocellular carcinoma(HCC) operation and 6 cases of normal hepatic tissue were chosen as control,all fixed with 10%formalin and conventionally embedded with paraffin.Each specimen was consecutively cut into slices of 4um thickness.Slices were stained by the monoclonal antibody of EGFR,EGFR mutations(del) and EGFR mutations(p) with SP immunohistochemical method.The expression of EGFR and EGFR mutations(EGFR mutations(del) and EGFR mutations(p)) in hepatocellular carcinoma tissue and their relationship with clinical pathology were analyzed with X2 test and Spearman's non-parametric analysis.Cox multi-factor regression analysis and Kaplan-Meier survival analysis were used to analyze the survival time of patients after operation and various parametersRESULTS:1.The positive rate of EGFR,EGFR mutations(del) and EGFR mutations(p) was 66.7%(4/6),0(0/6)and 0(0/6) respectively in normal hepatic tissue,and the positive rate of EGFR,EGFR mutations(del) and EGFR mutations(p) was 69.2%(54/78),21.8%(17/78) and 9.0%(7/78) respectively in hepatoma tissue.The expression of EGFR had no significance between normal hepatic tissue and hepatocellular carcinoma tissue(X~2=0.171,P=0.680),no expression of EGFR mutations was observed in the normal hepatic tissue.The positive rate of EGFR mutations(del) was higher than that of EGFR mutations(p)(X~2=4.924, P＜0.05).2.The expression of EGFR mutations was positively correlated with women(r=0.743,p＜0.05),and had a negative correlation with preoperative AFP value(r=-0.519,p＜0.05),hepatitis B(r=-0.567,p＜0.05), hepatic cirrhosis(r=-0.245,p＜0.05) and pathological grading (r=-0.273,p＜0.05);The expression of EGFR mutations(del) was positively correlated with women(r=0.453,p＜0.05),and had a negative correlation with preoperative AFP value(r=-0.243,p＜0.05),hepatitis B(r=-0.693,p＜0.05),hepatic cirrhosis(r=-0.328,p＜0.05) and pathological grading(r=-0.343,p＜0.05);The expression of EGFR mutations(p) in liver tissue was positively correlated with women(r=0.390,p＜0.05),and had a negative correlation with hepatitis B(r=-0.657,p＜0.05)and pathological grading(r=-0.347,p＜0.05).The remaining not-mentioned parameters were unrelated to the expression of EGFR and EGFR mutations.All of the above parameters had nothing to do with the expression of EGFR.3.Tumor pathological grading(P＜0.001) and preoperative AFP value(P＜0.001) were main influencing factors on postoperative survival time of patients with liver cancer in the group.Postoperative survival time had no significant difference between the patients with positive expression of EGFR and EGFR mutations and others with negative expression(P＞0.05).CONCLUSION:1.The expression of EGFR existed in normal hepatic tissue,the expression of EGFR mutations(del) and EGFR mutations(p) was not found in normal hepatic tissue,while EGFR,EGFR mutations(del) and EGFR mutations(p) all expressed in hepatocellular carcinoma.2.The expression of EGFR mutations and EGFR mutations(del) was positively correlated with women,and had a negative correlation with preoperative AFP value,hepatitis B,hepatic cirrhosis and pathological grading;The expression of FGFR mutations(p)in liver tissue was positively correlated with women,and had a negative correlation with hepatitis B and pathological grading.The remaining not-mentioned parameters were unrelated to the expression of EGFR and EGFR mutations.All of the above parameters had nothing to do with the expression of EGFR.3.The expression of EGFR,EGFR mutations(del) and EGFR mutations(p) in hepatocellular carcinoma was not an independent prognostic factor for patients with liver cancer.