Observation Of Experimental Rabbit Corneal Neovascularization By Suture-induced And Alkali Burn

Objective: To explore the difference of the experimental rabbit corneal neovascularization by suture-induced and alkali burn.Method: All animals(n=75)were randomly divided into three groups, normal control, consisting of 10 eyes(5 rabbits) ,the others each consisting of 35 eyes (35 Rabbits) . Rabbits corneal suture-induced and alkali burn models were established by routine methods. Biomicroscopic features including the length and areas of corneal neovascularization were observed .Immunoreaction of VEGF was detected on the 1st, 2nd,3rd, 5th, 7th, 10th an d 14th day respectively after operation , and the protein expressions were measured by image-analysis system.Results: Corneal neovaseularization began to obviously invade cornea on day 3 and flourished on day 7, then reached its developmental peak on day 10, and regressed gradually after day 14. Significant difference (P<0. 01) was found between the area of CNV by suture-induced and alkali burn. In CNV tissues ,the positive Immunoreaction of VEGF was most apparent during the 3rd～7th day and then declined remarkably on the 10th day .But the positive immune reaction of VEGF of suture-induced group was declined faster than alkali burn group.Conclusions: The method of alkali burn can induce more CNV and the CNV was declined slowly .The method of suture has better controllability of the area of CNV.