| PURPOSPURPOSE: To evaluate the effect of genistein(Gen) administration on cornealAlkali-induced corneal neovascularization (CNV) in rabbits.METHODS:One of 13 normal New Zealand white rabbits was used to observethe drug adverse reactions in the anterior chamber and other organizations. Theremaining 12 rabbits (24 eyes) were used of l mol / L NaOH infiltration applicated offilter paper(diameter=10mm) on the center cornea for 60 seconds, induced by alkaliburn in the formation of CNV, then were randomly divided into 4 groups asDay3,Day7,Day14 and Day21.Immediately after alkali burn on eyes , rabbits wereadministered by dimethyl sulfoxide (DMSO) solution on the left eyes(control) and andGen soluted by DMSO on the right eyes. Digital photographs of the cornea weretaken and analyzed to determine the area of the cornea covered by CNV on the day of3rd,7th,14th and 21st day after alkali burn. In addition,HE staining andimmunohistochemical analysis waer used to determine vascular endothelial growthfactor (VEGF) expression in corneal tissue after the rabbits were excuted.RESULTS: Analysis of digital photographs showed that there were less CNVGen-treated eyes than in controls (P < 0.001, compired t test). In addition, there wasless staining for VEGF in corneas from Gen-treated eyes than in control eyes. Gensolution drops and DMSO solution drops were not associated with any complicationsduring observation.CONCLUSION: Genistein administration could decrease alkali burn cornealneovascularization in rabbits,that provides a new way for clinic to treat cornealneovascularization. |
