Study On HPLC Fingerprints Construction And Medicinal Materials Quality Evaluation Of Cyathula Officinalis Kuan

Medicinal Cyathula Root is the dry root of Cyathula officinalis Kuan Plant,which is one kind of Perennial herb,Amaranthaceae,Cyathula Blume.Cyathula officinalis Kuan mainly distributes in Si'chuan,Yun'nan and Gui'zhou province.No matter wild or cultivated,most of them are growing in the districts above 1500m altitude.And especially the the areas of Si'chuan Tian'quan,Bao'xing,Han'yuan and Jin'kouhe own the maximun output.Recently,as a result of the degradation and hybridization of varieties,planting limit, changes of cultivation methods and other factors,there are huge heteromorphosis in the product qualities.Exact evaluation of the CyathuIa offcinalis Kuan plays a sinificant role in guiding scientific cuItivation,breeding purification and rejuvenation.Therefore,this paper established HPLC fingerprint building method for checking the quality of Cyathula offcinalis Kuan just by experimentation,and evaluated the whole quality of the samples, which are quite different in varieties,producing areas,planting limit,and so on.The evaluating results are as follows:1.Set the 4-year-old Cyathula officinalis Kuan from Jin'kouhe as a sample,the paper compared and optimized the chromatographic method which consist of column,detection wavelength,mobile phase,elution program,column temperature,and flow rate.And also obtained the best sample extraction method through analyzing the impact led by the different extraction solvents,method and conditions.As a result of the method validation, the retention times and relative peak areas of the main shared peaks had no significant changes in the test.Their RSDs were all less than 0.5%and 3.9%respectively.And the System stability and repeatability,equipment precision are all satisfactory.2.Using the optimized extraction methods and chromatographic conditions,studied 31 batchs of samples,which including 27 batchs collected from Tianquan,Baoxing, Yingjing,Jinkouhe and 4 batchs bought from dafeng market in chengdu.Using the optimized extraction methods and chromatographic conditions,studied 31 batchs of samples,which including 27 batchs collected from Tianquan,Baoxing,Yingjing,Jinkouhe and 4 batchs bought from dafeng market in chengdu.The chromatogram showed that the peaks were separated well and accorded with the fingerprint of the criterion.Among 31 samples 10 shared peaks were indentified,which also are special fingerprint peaks.Then, the author using the optimized extraction methods and chromatographic conditions,studied 31 batchs of samples,which including 27 batchs collected from Tianquan,Baoxing, Yingjing,Jinkouhe and 4 batchs bought from dafeng market in chengdu.The chromatogram showed that the peaks were separated well and accorded with the criterion of the Fingerprint,and the similarities are between 0.791 and 0.969,satisfactory.Among 31 samples 10 shared peaks were indentified,which also are special fingerprint peaks.And then,the Cyasterone peak was been located and the HPLC Fingerprint was been built up.3.The recult of the cluster analysis during the 31 batchs of samples shown,The quality of Cyathula officinalis Kuan certain regional characteristics,and most of samples from the same region are gathered in the same group.However,the 6 batchs from Lianglu distributed in 3 groups,maybe,their provenance were serious mixed.4.Cyasterone as a mark,in three-year Cyathula officinalis Kuan,the Bainiuxi (vernacular name,a kind of Cyathula officinalis Kuan) from Jingkouhe is the best, Baoxing followed,Yingjing is poor;the best Dabainiuxi and Hongniuxi are from Tianquan, the weakers are from Baoxing,and the worsts are from Yingjing.On all of the methanol-soluble composition,the three-year Bainiuxi and Dabainiuxi from Tianquan are the best,Baoxing followed,the worsts are from Yingjing;the three-year Hongniux from Yingjing has the best quality,followed by Baoxing,Tianquan is the most bad.5.All of the methanol-soluble composition as a mark,in Baoxing and Tianquan,the Bainiuxi is best,followed by Dabainiux,Hongniuxi is in the end;However,set Cyasterone as a mark,Binniux from Baoxing and Dabainiuxi from Tianquan are the best.6.Also,the qualities are different among different parts of the Cyathula officinalis Kuan root.Overall,in two-years root,Cyasterone and all of the methanol-soluble composition in lateral roots are the richest,followed by the two ends of the main root,the middle is slightly lower.In three-years root,Cyasterone in the bottom of main root is the highest.7.The author planted the seeds from different populations together at Xingou Tianquan.And the best quality appears from the Zishi(Tianquan) and Xiaogou(Tianquan) populations;after comparing and analyzing,the author also found that their ecological adaptability are so weak.And the living environments' change can cause the quality swinging.