| Traditional Chinese medicine Cyathula officinalis Kuan is common and genuine crude drug in Sichuan. It is the dry root of the plant Cyathula officinalis Kuan, which is a perennial herb belong to family amaranthaceae and genus Cyathula. It is clinically for the treatment of the blood stagnation, rheumatism and bruises. However, due to the extensive management of long-term cultivation,varieties degradation and mixed, quality of the drug decreased.1-. Resource survey in the genuine producing area of Cyathula officinalis Kuan Though field survey of three genuine producing areas, several data were got, like ecological environment, hybrid varieties, producing status and the sales price.The hybrid varieties was serious, traditional cultivation was abandoned. Now, the leading variety in producing areas are C.officinalis Kuan, C.capitata Mop and C. officinalis*C. capitata.2、Study on the genetic diversity of Cyathula officinalis Kuan6populations of Cyathula officinalis Kuan in Sichuan were preliminary evaluated. ISSR molecular maker was used in the study of plant traits and genetic diversity of6population and23samples. Though the primers screening test,4polymorphic primers were chose. The average amplified bands were10.75, a total of amplified bands were43, accounting for67.4%of the amplified bands number. Analysis shows in POPGENE, Cyathula officinalis Kuan had a wealthy genetic variation (at the species level He=0.1892, PPL=67.4%,Ho=0.3224; at the population level, He=0.717, PPL=49.16%, Ho=0.2509). The population genetic distance varied from0.52to1.00. In the main producing areas of Sichuan, the genetic diversity is lager in the Cyathula officinalis Kuan species and it is smaller in the population. The genetic diversity is mainly caused by the hybridization between C.officinalis Kuan and C.officinalis*C.capitata. 3、Study on the seed of C.officinalis KuanCurrently, at high altitudes the seed germination rate of C.officinalis Kuan is high, and it is significantly reduced at the altitudes fewer than1500m. The measured seed water content, viability, weight and health of the corresponding data can provide a valid basis for the seeds of C.officinalis Kuan the quality control. Through research C.officinalis Kuan seed coat permeability, Soaking24h the C.officinalis Kuan seed close to the suction saturation to ensure seed germination. Study hormones, soaking time and temperature the C.officinalis Kuan seed germination. Different concentrations of indole acetic acid to dealing with C.officinalis Kuan seeds, can significantly improve the germination rate and germination potential.4、Growth and microstructural studies of C.officinalis Kuan root Through research, our group found the different life C.officinalis Kuan in column in the share of the entire cross-section of the proportion of parenchyma cells containing calcium oxalate crystal sand, Cubic number; C.officinalis Kuan itshybrid varieties in the cork layer has a thickness and degree of cork, phelloderm the thickness juniors vascular ring number and the number of the forming layer thickness, xylem and the vascular system of the central secondary structure catheter pore sizeand arrangement, the number of transistors per unit area of thin-walled cells are different.5、Study on agronomic traits and flower morphology of C.officinalis Kuan By analysis on the different provenances C.officinalis Kuan agronomic traits, found of different provenances C.officinalis Kuan in the plant height, stem circumference, crown, leaf length, leaf width, plant fresh root weight, root length, root number of branches, perimeter of the root, dry discount rate significantly different. Flower morphology of different provenances C.officinalis Kuan anatomy and found that the different provenances C.officinalis Kuan the number of stamens, stamen color, shape of the stamens, ovules, dried flower color has some differences. 6。The quality evaluation on C.officinalis Kuan1) Determination of pol ysaccharide contentThrough research, to establish a method for the determination of polysaccharide in C.officinalis Kuan found the same years C.officinalis Kuan polysaccharide content miscellaneous C.capitata Mop higher C.officinalis*C.capitata; With the increase of years, C.officinalis Kuan polysaccharides the accumulation is also increasing.2) Determination of Cyasterone32batches of C.officinalis Kuan to determine the content, the average three-year-C officinalis Kuan Cyasterone content of0.065%, C.officinalis*C.capitata Cyasterone content of0.057%, the C.capitata Mop Cyasterone content of0.033%, significantly lower than C.officinalis Kuan and C.officinalis*C.capitata. With the increase of years,1year the students C.officinalis Kuan average Cyasterone content is only0.020%,0.031%and2-year-old less than0.030%.3years old,4years the raw Cyasterone contents were0.058%,0.061%. Description With the increase in life the Cyasterone of accumulation also increased to three years to stabilize.3) Fingerprinting of C. officinalis KuanBy32batches C.officinalis Kuan samples to establish a schematic diagram of the calibration of13common peaks, identified the no.4peak as the peak of ferulic acid peak,the no.5peak as the peak of Cyasterone, the no.10peak as the peak of oleanolic acid,wherein the no.4peak as the reference peak, found C.officinalis Kuan, C.capitata Mop, C.officinalis*C.capitata fingerprints are very different. C.officinalis*C.capitata, C.capitata Mop unique peak T1, T2average peak area of the total peak area were15.3%,19.6%. It is indicate that those Chemicals on the entire C.officinalis*C.capitata, C.capitata Mop chemical composition had a very large impact.7、Quality of C.officinalis Kuan multi-factor correlation analysisGrey Relational Analysis on the above Cyasterone content, genetic characteristics, ecological environment, agronomic traits, microscopic characteristics. Results Cyasterone transhipment and transport of ferulic acid accumulation and leaf has a positive addition (Leaf width> leaf length> parcels filling> plant fresh root> stem circumference> the root branch> root length> height> root perimeter).It may also be influenced by photosynthesis, has important links with the altitude(The altitude> annual precipitation average relative humidity> annual evaporation annual average sunshine>10℃effective temperature average annual temperature) There are also important links with precipitation; with thickness of phelloderm has important links(Thickness of the inner layer> the cork layer thickness> Sansei number of vascular bundles of calcium oxalate crystals number), the prompts Cyasterone and ferulic acid accumulation in phelloderm. |
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