The Effects Of Saponins Of Rhizoma Polygonati On The Mouse Model Of Depression And Its Mechanism

Objective : After establishing the acute stress, drug-induced and chronic stress mouse models of depression,we observed the effects of Saponins of Rhizoma Polygonati(SRP), on behaviors of all the models. And studied on the effects of the SRP on norepinephrine(NE), dopamine (DA), 5-hydroxytryptamine(5-HT), and cortisol(COR) in mouse model of depression with chronic stress. Then discussed the prevention and cue effects of the SRP and the possible mechanisms.Methods: 1. Established different depression models of mouse in different ways :⑴In this study, we adopt the tail suspention and force swimming of the mouse of two" the behavior despair" to observe effect of the SRP on suspention and force swimming immobility time of the mouse.⑵In this study, we set up the drug-induced depression model by reserpine, and then observed the ptosis, akinesia and hypothermia which induced by reserpine.⑶The depression model of mouse was made by the CUMS and solitary custody. We observed the change of the weight of the mouse, and completed the detection of autonomous activity, open-filed test, and water maze test.2. Using the High Performance Liquid Chromatography(HPLC) detected the monoamine neurotransmitters of the CUMS depression model; and deteced the COR with the radioimmunity assay (RIA).Results: 1,⑴The SRP(100,200,400 mg·kg-1) significantly diminished the immobility time of the mice in the suspention and force swimming.⑵After administration of the SRP for 7 days, it can notably alleviate the symptoms of the ptosis, akinesia and hypothermia which occurred in the vehicle group.⑶The SRP(100,200,400 mg·kg-1) all increased the weight of the mice; The numbers of crossings and rearings in the open field test and the autonomous activity were increased as well. According to our findings of water maze , the SRP(100,200,400 mg·kg-1) shortened the Escape latent period from the second day obviously, and prolonged the cross the Platform Quadrant times.2,The SRP(100,200,400 mg·kg-1) significantly increased the NE and 5-HT in the brian of the CUMS depression model mouse; the contents of the DA had rising trend; and the COR in the serum decreased.Conclutions: The SRP can improve the diversify of the behaviors, elevate the contents of the NE, DA and 5-HT in the brian and the COR in the serum which are the possible mechanisms of the prevention and cure effect of the SRP.