| Cyclophosphamide, with broad-spectrum anti-cancer effect, is one of the significant alkylating agents to treat cancer. But some serious problems of clinical medication such as the toxic side-effects like liver damage, bone marrow suppression and immunosuppression are prominent. So look for the attenuated synergies with drugs and joint application, in order to prolong the service life of cyclophosphamide and improve the medicinal value become the urgent matter.Rhizoma polygonati, a common Chinese medicinal herb, was first recorded in Medicine Classification. It is natured and sweet, which can invigorate the function of the spleen, benefit the kidney, moisten the lung, reinforce qi and nourish yin. The chemical composition of this medicine includes rhizoma polygonati polysaccharide, anthraquinone compounds, trace elements and wooden fat element, steroidal saponins, alkaloids, etc. According to the modern medicine research, phizoma polyonati is considered to have the functions of protecting organs, optimizing immune response, lowering blood pressure, as well as playing anti-oxidation and anti-tumor function. So this topic chooses yellow with cyclophosphamide combination, Rhizoma polygonati on cyclophosphamide attenuated synergistic effect, so as to essence in cancer adjuvant chemotherapy and Rhizoma polygonati as regard to the development of antineoplastic drugs provide some experimental basis.Objective:Research on attenuated synergies of cyclophosphamide by rhizoma polygonati to provide theoretical basis for the further development and clinical application of rhizoma polygonati(RP).Methods:Recovery sarcoma S180 cells need to be diluted with physic- ological saline.Then mice were inoculated intraperitoneally for subculture of the cells.The ascites would be drawn seven days later under sterile conditions to allocate cell suspension(at a concentration of 1.75×107 tumor cells/m L).50 mice were randomly divided into control group, a tumor-burdened group,CTX group,CTX+low-dose Rhizoma polygonati essence group,CTX+high dose group,each mouse was injected with 0.2 m L cell suspension under its right armpit. One day after inoculation, the 50 mice were numbered and weighed, then randomly divided into five groups, naming the normal group, the tumor-bearing group,CTX group,CTX+medium-dose RP group,CTX+ high- dose RP group. The second day of modeling, the mice were administrated drugs,which lasted for 11 days.On the next day after stopping administration, all 50 mice had to be killed for observing their general condition, body weight and food intake.Then the inhibition rate, kidney index, spleen index, thymus index,the content of superoxide dismutase(SOD), malondialdehyde in liver tissue(MDA),glutathione peroxidase(GSH-PX) were calculated. The changes of indicators such as heart,liver and kidney function, peripheral blood, peritoneal macrophages function,bone marrow nucleated cell counts,T lymph- ocyte proliferation,and NK cell activity were monitored.Results:The inhibition rate of CTX + medium- and high-dose RP groups were significantly higher than that of CTX group(P<0.01).The body weight and food intake of CTX + medium-and high-dose RP groups were higher than that of CTX group.The thymus index of CTX group, CTX+high-dose RP group and tumor-bearing group were significantly different(P<0.01), meanwhile the thymus index of CTX+medium-dose RP group and tumor- bearing group was also different(P<0.05);The spleen index of CTX group and tumor-bearing group were significantly different(P<0.01),at the same time the spleen index of CTX group and CTX+medium and high-dose RP groups were also different(P<0.01).Compared with the normal group,the kidney index and serum urea nitrogen(BUN) of the mice in the other groups were increased with statistical significance(P<0.05), the kidney index and BUN of tumor- bearing group and CTX group were significantly different(P<0.05), the kidney index and BUN of CTX group and CTX+medium and high-dose RP groups were also different(P<0.05);compared with the normal group,the values of aspartate aminotransferase(AST), alanine aminotransferase(ALT) in other groups were increased with statistical significance(P<0.05-0.01),while compared with CTX group, the values of AST and ALT were decreased without statistical significance(P>0.05);compared with the normal group, MDA values of tumor-bearing group were increased with statistical significance(P<0.01),MDA values of CTX + medium-dose RP group and CTX group was significantly different(P<0.01);Compared with the normal group, the GSH-PX vitality of the tumor-bearing group was significantly declined with statistic significance(P<0.05), the GSH-PX vitality of CTX group and CTX+medium and high-dose RP groups were significantly different(P<0.05); compared with CTX group, glutathione peroxidase(GSH) of the tumor- bearing group and CTX+medium and high-dose RP groups were significantly different(P<0.05).Compared with CTX group, the number of peripheral leukocytes, platelet and bone marrow cells of CTX+medium and high-dose RP groups were significantly increased(P<0.01-P<0.05).Compared with CTX group, the phagocytosis of peritoneal macrophages, T lymphocyte proliferat- ion and NK cell activity of CTX + medium and high-dose RP groups were significantly increased(P<0.05).Conclusion:Experiments show that demonstrated RP enhances antitumors effect of CTX,indicated the use of RP improves the mice’s quality of life,showed RP can protect the spleen and thymus of the tumor-bearing mice as well as promote their growth and reduce the toxic effects of CTX on their immune organs,the above indicators show that RP can reduce the damage of tumor-bearing mice’s hearts and livers caused by CTX,suggesting that RP can fight against the inhibition of CTX on bone marrow,indicating that RP can improve the phagocytosis of peritoneal macrophages and relieve the damage of macrophage by chemotherapy and improves T lymphocyte proliferation and NK cell activity. Rhizoma polygonati(RP) showed synergism and attenuation actions with cyclophosphamide(CTX). |
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