| Objective:Lipoprotein - associated Phospholipase A2,(LP-PLA2),Also known as platelet-activating factor acetylhydrolase water activity, which is a class of phospholipid glycerol in the part of the Sn - 2 fracture sites ester selective enzyme. LP-PLA2 widely distributed in the biological, especially in mammals, reptiles, and arthropods cells, tissues and fluids can be separated.Its sources include:the pancreas, stomach, heart, bowel, spleen, liver, lungs and brain, cartilage cells and vascular smooth muscle cell nucleation, white and macrophages, red, platelets, pancreatic juice, inflammatory exudate, ascites and seminal plasma. LP-PLA2 in human plasma mainly composed of mature macrophages, T lymphocytes, mono- cytes, and mast cell secretion with glycerol phospholipids generated activity of enzymes break down the family. The production by its mediated the lipid medium such as prostanoid material, leukotrienes, were lysophospholipids platelet activate factor and so on. Which with the function of phospholipids transhipment, film repair, extracellular hydrolysis and neurons release of transfer factor and so on in many physiological and pathological conditions. Thus, this kind of enzymes and mediated product recently attracted great attention.LP-PLA2 physiological functions including phospholipids structure, to promote the necrotic tissue autologous disappear. Participate in alveolar surface active substances metabolism, etc. In the decomposition, digestion, phosphatide acyl off or then acylation and balanced cell phospholipids pool processplay an important role. produced by arachidonic acid primarily free fatty acid and were lysophospholipids. Inflammation occurs, LP-PLA2 activity, through the activation ring oxidase, lipoxygenase, further produce large amounts of 20 carbon silane acid material, such as prostaglandins, thrombosis, and platelet activation leukotriene proinflammatory cytokines factor etc. These substances through their respective function approach, continue to induce more proinflammatory cytokines, forming a cascade falls, promote the development of effect of inflammation. Therefore, LP-PLA2 will damage factor, inflammatory cells, lipid neurotransmitter, cytokines cells visible part and soluble molecules, become the system key active.Recent epidemiological and clinical prospective studies have found that Occurrence of coronary heart disease events compared with the control group or the quality of LP-PLA2 activity was significantly increased, so that the quality of plasma,or LP-PLA2 activity increased the risk of cardiovascular events is an independent risk factor.LP-PLA2 hydrolysis oxidation lecithin generated hemolysis lecithin and oxidation type free fatty acid is medium, can stimulate proinflammatory cytokines factor and adhesion, thus promote mononuclear cells lining by lumen to gather. After endometrium mononuclear cells in gathered to macrophage derived and macrophage cell oxidation type LDL into foam cells. Foam cell aggregation of atherosclerotic plaque, vulnerable plaques of rupture, easy cause thrombosis and cardioascular eents. Therefore showed a strong urge the role of atherosclerosis, clinical and laboratory findings confirm it.This research through the detection of diabetes plasma lipoprotein related phospholipid A2 enzyme activity level in type 2 diabetes patients lipoprotein related phospholipid A2 distribution and influencing factors.Materials and methods: Using case-control study, A Chinese han nationality 230 cases of type 2 diabetes. Vascular ultrasound according to the neck, the result of atherosclerotic plaque atheromatous plaque, Divided into atherosclerosis group (125cases), non-atherosclerotic group (105cases), Determination of all patients into the height, weight, calculated body mass index.Determination of liver biochemistry, renal function, blood lipids and other biochemical parameters of conventional. Collecting blood samples, using immunohistochemical double-antibody sandwich ELISA method in the determination of plasma LP-PLA2 activity, use t-test etc statistical methods processing data, analysis between two groups of enzyme LP-PLA2 activity distribution characteristics. And further analysis of lipid meta- bolism disorders, age, gender, obesity, postmenopausal, masculine smoking to this enzyme activity distributed influence.Results:LP-PLA2 activity in our country type 2 diabetics: Atheros- clerosis group to be higher than without atherosclerosis group. The male is higher than the female. After the menopause, the feminine group is not higher than the menopause female. The obese patient is higher than the non-obese patient; Low clearance high density lipoprotein is higher than the high density lipoprotein normal person. Between hing clearance low density lipoprotein and the low density lipoprotein normal person not statistically significant differences . Between full 60 years old and under 60 years old not statistically significant differences .Men between smoking and non-smokers not statistically significant differences.Conclusion:Chinese of the Han nationality patients with type 2 diabetes, the trunk pathological change, has atherosclerosis group, the blood plasma Lp-PLA2 activeness is high, Sex, obese and fat metabolic disorder particularly low high density lipoprotein, that affect patients with type 2 diabetes plasma Lp-PLA2 activity in the relevant factors.
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