Clinical Significance Of Dendritic Cells Infiltration And Surface Molecules Expression In Breast Cancer

Objective: To investigate the co-relationship between the number and function of tumor infiltration of dendritic Cell and Bio-behavior and cure of breast cancer, by analyzing the number of DC and the expression of its surface molecules in Breast Cancer tissue and tissue nearby. Immunity suppressor factor released by cancer cell i.e. IL-10, IL-12, TFG-β1 VEGF and so on reacts on DC and results in a change of the expression and function of its surface molecules. The single or joint reaction of Immunity suppressor factor may cause the low expression or non-expression of the CD surface molecules, reduce the function of professional antigen presenting cells, and lead to immunity escape. This result is very useful to grasp the change in the number and function of CD in cancer cells, protect the function of CD, and improve the effect of immune therapy. At the same time it provides important basis for clinical usage of target tissue gene therapy.Methods: Material taking and hematoxylin and eosin (HE) staining: Take the breast cancer tissue and the adjacent tissue in 30 minutes after the modified radical mastectomy.Keep them in -80℃refrigerator cold storage. After paraffin-embedded, make the tissue into sections of 3 microns. Then Hematoxylin and eosin stained; Detection of the expression of dendritic cells in breast cancer and the surrounding tissue in the method of immunohisto- chemistry: detect the expression of dendritic cells in breast cancer and surrounding tissue by Quick-use MaxVisionTM Immunohistochemical detec- tion kit. Analysis the degree of the dendritic cells expressed in breast cancer and the adjacent tissues.Investigate the relationship between the expression of the dendritic cells and the invasion depth, size and metastasis of the breast cancer tissues; detection of the expression of IL-10, TGF-β1, VEGF in the mRNA level in breast cancer and adjacent tissue by semi-quantitative RT-PCR method: Take about 50 mg breast cancer tissue, use TRIZOL to extract the total RNA, reverse transcription into generate cDNA using Oligo-dt15 as the primer. Use the product cDNA as the template and the corresponding primers to PCR amplify. Detect the PCR products by 2% agarose gel electrophoresis. Detect the expression of IL-10,TGF-β1,VEGF in the mRNA level of the adjacent tissue in the same way.the primer is designed by Primer5 primer design and Oligo primer design software and is synthesisded by TaKaRa Biotechnology (Dalian) Co., Ltd.Results: Among the 26 cases, positive dyed CDs using Immunohisto- chemistry method in the breast cancer tissue mainly spread around the cancer cells, and a few of them spread in or between the cancer cells. Their pseudopodium touches the cancer cells accompanied by lymphatic infiltration. The statistics result reveals that the number of CDs in breast cancer is obviously less than that in the normal tissues near the breast cancer; In the breast cancer tissue all of the 26 cases have a positiveβ-actin expression.the positive rate is 100%.20 cases have a positive IL-10 expression, the positive rate is 76.9%.20 cases have a positive TGF-β1 expression, the positive rate is 76.9%.17 cases have a positive VEGF expression, the positive rate is 65.4%.While in the adjacent tissues ,only 3 cases have a weak positive VEGF expression in mRNA level the positive rate is 11.5%.7 cases have a weak positive TGF-β1 expression and weak positive IL-10 expression in mRNA level, the positive rate is 26.9%. All of the 26 cases have a positiveβ-actin expression,the positive rate is 100%.the positive expression rate in the breast cancer tissue is significantly higher than that in the adjacent tissues.the difference is statistically significant (P <0.05); Among the 26 cases ,5 cases have cells which express CD1a~+ and CD83~+ in the breast cancer tissue.None expresses CD54 among the 0 detected cases. The expression of the molecules on the surface of the dendritic cell is significantly depressed.Conclusion: The reduction of the the dendritic cells number in invasive breast cancer tissue result in lower immune function and may be one reason of tumor occurrence and development; The low expression or even non- expression CD1a,CD83,CD54 surface molecules on the dendritic cells in the breast cancer tissue suggest that tumor-infiltrating dendritic cell is a kind of low function or non-function DC which is unable to effectively identify and kill the tumor cells; A large number of breast cancer cells in local breast cancer organizations excrete immunization inhibiting factor such as IL-10,TGF-β1 and VEGF. And may be one of the reasons why the MHCⅠ,Ⅱmolecules on the surface, costimulatory molecules,immune adhesion molecule low express through individual or joint action. Thereby,reduce the antigen- presenting function of the dendritic cells,leading to immune escape of tumor cells and promote tumor growth.