| Cynoglossum amabile Stapf et Drumm is a traditional herb in china which belong to family Cynoglossum(Boraginaceae) and widely distributed in Xin Jiang, Gui Zhou, Guang Dong, Guang Xi, Yun Nan, et al. It is described as cool-natured,bitter in taste and has functions of clearing away heat evil and promoting diuresis,eliminating stasis to activate blood circulation hematischesis and analgesia, et al and is used as an anti-inflammatory, antihepatitis, anticystitis agent in Chinese folk medicine. It is very important and instructive to study the chemical constitents systematically and establish the quality standard of active components .In this thesis, a systematical investigation of the chemical constituents from the the herbs of Cynoglossum amabile Stapf et Drumm was carried out and 10 compounds were separated by using open-column, gel chromatography ,preparative TLC methods and recrystallisation et al. Theirs structures were elucidated by means of IR, MS, NMR and chemical methods respectively. They were elucidated as Stigmasterol(1), Octacosanoic acid(2),β-sitosterol(3), Daucosterol(4), O-Hydroxybenzoic acid(5), 3,4-Dihydroxybenzoic acid(6), 2α,3β-dihydroxyolean-12-en-23,28,30-trioic acid(7), 2α,3β,19α,23-tetrahydroxy olean-12-en-28-oic acid(8), 21-O-β-D-glucopyranosyl- 2α,3β,21β,23-tetrahydroxyolean-12- en-28-oic acid(9) and (2S,2'R,3S,4R,12E)-N-(2'-hydroxytetracosanoyl)-2-amino-octadec- 12-ene-1,3,4,-triol(benjaminamide, 10) Compound 7 was a new cmpound,named as amabilic acid, and compounds 2, 5, 6, 8, 9, 10 were obtained from this genus for the first time.The bioactivities of compound 7 of anti-inflammatory and antitumor were carried out, the result indicated that amabilic acid had an obvious effect of anti-inflammatory activity.
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