| Objective In this experiment, we construct a rat model of Adriamycin-induced glomerulosclerosis and observe the effect of tranilast on the pathology change of kidney and the expression of CD147 and MMP-2 . And also we discusse the mechanism of Adriamycin-induced glomerulosclerosis in rats and provide some evidence for its prospective use in treatment of glomerular fibrosis.Methods Thirty Sprague-Dawley male rats were randomly divided into sham operation group(S group) and operation group. The operation group rats underwent unilateral nephrectomy of left kidney and intravenous injection of doxorubicin. Then the operation group rats were randomly divided into two groups: model group(M group), tranilast-treated group(T group). T group rats were fed tranilast(400mg/kg/day) and then albuminuria, creatinine, urea nitrogen, were detected. The rats were killed eight weeks later and the right renal tissues were examined by microscope. And the expression of CD147 and MMP-2 was studied by PT-PCR and immunohistochemistry. The intensity was analyaed by imagine quantitative analysis technique.Results (1)PAS staining of the renal tissue revealed that S group rats had no obvious pathological changes. M group rats tissue mainly revealed that mesangial cells mild to moderate segmental hyperplasia, extracellular matrix significantly increased, focal segmental sclerosis, and that a small portion of the ball sclerosis, capillary cavity collapse of the segmental renal weight / body weight significantly increased(P<0.05), glomerulosclerosis index increased significantly(P<0.05). Compared with M group, T group pathological changes reduced significantly, a few glomerular mesangial cells and stromal mild to moderate segmental increased capillary cavity see only a few mild stenosis, and occasionally focal segmental sclerosis, kidney weight / body weight compared model group decreased(P<0.05), glomerulosclerosis index decreased significantly(P<0.05). (2) Immunohistochemistry showed that group M CD147 and MMP-2 expression decreased, compared with the S group statistically significant(P<0.05), obove expression increased in the T group(P<0.05). (3) RT-PCR showed that in the M group CD147 and MMP-2 mRNA expression, compared with the S group statistically significant(P<0.05), obove expression increased in the T group(P<0.05).Conclusions (1) In the doxorubicin rat model of renal sclerosis, tranilast can reduce the renal pathological damage and delay the process of glomerulosclerosis. (2)Tranilast can inhibit the expression of CD147 and MMP-2 mRNA and protein. (3) Effects of tranilast on glomerular sclerosis may be related to the preventive effect of increased expression of CD147 to enhance the expression of MMP-2.
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