Studies On Chemical Constituents Of Propolis From Mountain Tai

Propolis is a natural resinous hive product collected by honeybees from leaf buds and exudates of various plant sources. It is composed of colophony, beeswax, a small quantity of volatile oil and pollen. The chemical constituents of propolis are very complicated. Up to now, researches indicate that the main active constituentsofpropolis are flavanoids, aromatic acid and aromatic acid esters, phenols, alcohols, aldehydes, ketones, terpenes and so on . The chemical constituents of propolis lie largely on the plants collected by honeybees. Because of the distinction on kinds of plants in different areas, the chemical constituents of propolis are appear vary greatly, which lead the chemical constituents of propolis from different areas may be totally different. So there is a very clear regional in the chemical constituents of propolis. In order to to carry out the quality supervisory control of propolis produced in one area, must go along researching on the propolis. This paper was first to carried out researching the chemical compositions and their quantitive analytical methods of propolis colleeted from Mountain Tai. Fifteen compounds are separated and appraised, and seven kinds of them for reference substance, and determined the differences of propolis contents between Mountain Tai and Guang zhou, then provide a basis for further active appraisement and establishing fingerprints of propolis. The results are as follows:1. By diethyl ether extraction and SPME collected vcolatile compounds which was analyzed and appraised by GC-MS spectroscopy techniques, and checked out seventy-four compounds. There were many differences in breed and content of the compounds obtained by the two methods. After comparing and analysing, the SPME was better in extracting volatile oil.2.The paper studied the ultrasonic extraction process, and established colorimetry which determined the total flavonoids in propolis. On this basis, the test studied the factors which affected the extraction rate of the active ingredients of propolis, and obtained the optimum extraction condition is: extracted 10min by ultrasound in 95% ethanol, at 35℃. Extracting by ultrasound can be obtained a higher efficiency compared with the industrial extraction.3. The extract is extracted by ultrasound, concentrated under reduced pressure, separated and purified by various means including solvent partition, silicagel column chromatography, thin-layer chromatography, recrystallization, HSCCC, Sephadex chromatogram. Fifty compounds be obtained from acetic ester extractive, and defined structures by UV and MS/MS~n, and appraised five flavonoid compounds, ten organic acids and esters.4. A HPLC method for the analysis of seven representative bioactive components: 1. Kaempferol 2.Alpinetin 3. tectochrysin 4.Benzyl p-coumarate 5. Benzyl ferulate 6.Caffeic acid phenethyl ester 7. Phenethyl p-coumarate in propolis was established, two samples form Mountain Tai and GuangZhou was analysed. It indicated that all the seven bioactive components are mutual constituents in two propolis samples, and there are significant differences between the propolis content from Mountain Tai and GuangZhou.