Cyclooxygenaseg-2 Expression In Epithelial Ovarian Carcinoma And Its Rejulation To Tumor Angiogenesis

Aim: This study aims to explore COX-2 expressio in epithelial ovarian carcinoma and normal ovary. Although further studies are the contributions of COX-2 to vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF) and MVD in epithelial ovarian carcinoma. We expect the research could detect the mechamism in epithelial ovarian carcinogenesis. Thus, it may offer effective prevention and therapy in human epithelial ovarian carcinoma.Methods: The COX-2 expression were examined in eighty matched sets of epithelial ovarian carcinoma specimens and eighteen normal ovary tissues, using immunohistochemistry, and its contributions to the expression of VEGF,bFGF and CD₃₄ also were investgated using immuneohistochemistry. We used weidner analysis system to assess the MVD as a percentage of the endothelial area, and the MVD was assessed using CD₃₄ immunohistochemistry.Results: (1)High COX-2 expression was found in epithelial ovarian carcinoma. There was significant difference among epithelial ovarian carcinoma and normal ovary(P<0.01); The COX-2 was not expressed in normal ovary. The COX-2 expression in epithelial ovarian carcinoma was significantly different in FIGO grades(P<0.01), and COX-2 expression was not correlated with histological types (P>0.05). (2) VEGF and bFGF were expressed highly in epithelial ovarian carcinoma. There were significant difference amongepithelia epithelial ovarian carcinoma and normal ovary(P<0.01); VEGF and bFGF were not expressed in normal ovary. (3) The expression rates of COX-2,VEGF,and bFGF were 85.0%(68/80),78.75%(63/80) and 75.0%(60/80), respectively; The expressien rates of VEGF and bFGF were 95.59%(65/68) and 92.65%(63/68) in positive group of COX-2 and the expression of VEGF and bFGF were not expressed in negative group of COX-2. COX-2 was significantly correlated with VEGF and bFGF. ( 4 ) MVD was (54.21±16.61) and (36.21±10.21) in positive and negative group of COX-2 respectively,and COX-2 was significantly correlated with epithelial ovarian carcinorma MVD (P<0.01).Conclusions: (1) High COX-2 expression was found in epithelial ovarion carcinoma, and it play an important role in tumorgensesis and development of epithelial ovarian carcinoma. (2). COX-2 is correlated with VEGF,bFGF and MVD using CD₃₄ staining, and high COX-2 expression is correlated with tumor angiogenesis in epithelial ovarian carcinoma. COX-2 can stimulate tumor angiogenesis and up-regulate invasion and metastasis in epithelial ovarian carcinoma (3)Synergistic exploring of COX-2,VEGF and bFGF is a useful progress.