Expression Of PDCD10 And Its Clinicopathological Significance In Gastric Cancer And Esophageal Cancer

Objective:Gastric cancer and esophageal cancer are digestive tract tumors whose morbidity and mortality are most common in China.The development of gastric cancer and esophageal cancer not only refers to the activation of proto-oncogene and the inactivation of tumor suppression genes,but also correlates with apoptosis genes closely.Programmed cell death factor 10(PDCD10) is a new apoptosis gene which originally be cloned by Peking University.Our study was aimed to evaluate the expression of PDCD10 protein and its clinicopathological significance in the pathogenesis and development of gastric cancer and esophageal cancer.The relationship between the expression of PDCD10 and the clinicopathological characters of gastric cancer and esophageal cancer was also analyzed so as to explore the effectiveness of PDCD10 in the diagnosis and prognosis of gastric cancer and esophageal cancer patients.Methods:The expression of PDCD10 was detected by SP immunohistochemistry method in human tissues including 26 cases of normal gastric mucosa,22 cases of chronic atrophic gastritis with intestinal metaplasia,66 cases of gastric carcinoma and 20 cases of normal esophageal mucosa,71 cases of esophageal squamous cell carcinoma.Results: Chapter1.Expression of PDCD10 and its clinieopathological significance in gastric cancer:PDCD10 expressed in cytoplasm of stomach glandular epithelium cells mainly. The positive rates of expression in normal gastric mucosa,chronic atrophic gastritis with intestinal metaplasia and gastric carcinoma were 11.5%,31.8%and 63.6% respectively,which was up-regulated progressively during the course(P＜0.05). PDCD10 protein expression in gastric carcinoma tissues was significantly higher than that in normal gastric mucosa and chronic atrophic gastritis with intestinal metaplasia. No significant relationship was detected between the expression and patients' sex,age, and the site of cancer(P＞0.05).The positive rates in patients with poorly-differentiated cancer were significantly higher compared with that in moderately and highly differentiated cancer(P＜0.05).The expressive level of PDCD10 protein in patients with positive lymph node and distant metastasis was significantly greater than that in patients without lymph node and distant metastasis (P＜0.05).Chapter2.Expression of PDCD10 and its clinicopathological significance in esophageal cancer:PDCD10 mainly expressed in cytoplasm of esophageal squamous cells.There was a significantly higher positive percentage(43.7%) of PDCD10 expression in esophageal squamous cell carcinoma than that in normal esophageal mucosa(10.0%, P＜0.05).The expression does not correlate with patients' sex,age,and the site of cancer(P＞0.05).In the esophageal squamous cell carcinoma,the positive rates of highly,moderately and poorly differentiated tissues were 20.0%,40.6%,62.5% respectively,which was up-regulated.The positive rates in patients with poorly-differentiated cancer were significantly higher than that in highly differentiated cancer(P＜0.05).And the positive rates in patients with lymph node and distant metastasis also were significantly higher than that in patients without lymph node and distant metastasis of esophageal cancer(P＜0.05).Conclusions:1.PDCD10 expresses in cytoplasm of stomach glandular epithelium cells and esophageal squamous cells,but the positive expression rates are low. 2.PDCD10 protein expression was up-regulated progressively during the course of normal gastric mucosa,chronic atrophic gastritis with intestinal metaplasia and gastric carcinoma.The positive expression percentages in gastric carcinoma tissues were significantly higher than that in normal gastric mucosa and chronic atrophic gastritis with intestinal metaplasia.This indicated that apoptosis mediated by PDCD10 played an important role in the development and progression of gastric cancer.And there was a correlation between the expression of PDCD10 and histological grade and metastasis of gastric cancer,which indicated that PDCD10 could be used as a molecular biology mark in gastric cancer patiens' diagnosis and prognosis.3.PDCD10 protein expression in esophageal squamous cell carcinoma tissues was significantly greater than that in normal esophageal mucosa,which demonstrated that abnormal expression of PDCD10 had a close relationship between esophageal cancer's development and progression.And there was a correlation between the expression of PDCD10 and histological grade and metastasis of esophageal cancer, which investigated that PDCD10 could be used as a molecular biologic mark in the diagnosis and prognosis of esophageal cancer patients.