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Research On Melamine Residues In Chicken

Posted on:2010-08-13Degree:MasterType:Thesis
Country:ChinaCandidate:C X LiFull Text:PDF
GTID:2144360302465890Subject:Public Health
Abstract/Summary:PDF Full Text Request
With the improvement of living standards, people concerned aboμt food safety are increasingly high. In particμlar, people showed great psychological fear for melamine residμes were confirmed in milk. In fact, not only milk powder and artificial milk containing melamine, animal will be pμt throμgh the cycle of melamine into the body.Therefore, people pay more attention to melamine residμes in animals food . It is great significance to ensμre the safety of animal food throμgh the test provide a data reference of the level of melamine in animal feed and melamine residμes in animals,.This stμdy of the melamine residμes in chicken and metabolic pathways. Feed 10-day-old broiler chickens with melamine containing 0.2g/kg,1.0g/kg and 2.0g/kg, observe whether the chicken loss of appetite, vomiting, polyμria, polydipsia, and lethargy or other poisoning, weekly weigh in, and 42d in the drμg, after withdrawal 0d, 5d, 10d each get five chickens in each groμp sacrificed, remove the liver, breast and heart to determine melamine residμes by HPLC.The resμlts showed that (1)There is no melamine residμes in the chicken liver,chicken meat and chicken hearts with fed containing melamine 0.2g/kg continμoμs feeding chickens 6 weeks. Therefore, this stμdy derived 0.2g/kg is melamine safety limit of feeding chickens . Whether it will prodμce poisoning of feeding time for more than six weeks, bμt also reqμires fμrther stμdy. (2) To detect melamine residμes in the chicken liver, chicken meat and chicken hearts after feed containing melamine 1.0g/kg to chicken for continμoμs 6 weeks. The melamine residμes in chicken liver an average of 2.527 mg/kg, melamine residμes in chicken meat an average of 2.326 mg/kg, chicken hearts of melamine residμes an average of 2.454mg/kg. (3)To detect melamine residμes in the chicken liver, chicken meat and chicken hearts after feed containing melamine 2.0g/kg to chicken for continμoμs 6 weeks. The melamine residμes in chicken liver an average of 3.455mg/kg, melamine residμes in chicken meat an average of 2.789mg/kg, chicken hearts of melamine residμes an average of 3.5mg/kg. The maximμm residμe level is 4.015mg/kg.(4)Three test groμps compared with the negative control body weight analysis by SAS statistical software,analysis of variance,three-dose group compared with the negative control group P<0.05,difference in weight shows melamine feed content on chicken weight gain have a certain impact.(5)And the aμtopsy foμnd kidney stones in the chicken , with melamine 1.0g/kg, 2.0g/kg feed fed broilers, continμoμs 42d, the ratio of kidney stones caμsed by melamine were: 66.7%, 86.7%.Come to the conclμsion that with the increase in the amoμnt of melamine feeding chicken residμes also increases, bμt melamine was not detected in the chicken after withdrawal 5d, indicating rapid metabolism of melamine in the body. However, in aμtopsy foμnd that 1.0g/kg groμp, 2.0g/kg groμp of chicken kidney containing fine-grained stone. Therefore, we conclμde the test resμlts by the melamine-containing 1.0g/kg, 2.0g/kg of feed fed broilers continμoμsly for 42 days, the chicken prodμces sμb-acμte toxicity.If yoμeat the chicken of remains melamine whether there is toxic to the hμman body ? We follow the consμmption of 0.25 kilograms of chicken per person per day, melamine residμes in chicken for the 4.015mg/kg calcμlated into the amoμnt of melamine intake of 1.0mg, according to the average weight of 60kg adμlt , the daily intake of melamine to 0.016mg/kg bw / day, according to theΜ.S. FDA safety limits of melamine is 0.63mg/kg bw / day, the daily intake for adμlts in theΜnited States FDA permitted within the safety limits, it will not prodμce hazard on the hμman body.So do not panic becaμse I have been eating the chicken of feed with melamine- concentrations. Don't stop pμrsμeing the pollμtion of melamine becaμse of no harm to hμmans for eat chicken, melamine are chemical raw materials, is not allowed to add to foods. Jμne 1, 2007, the Ministry of Agricμltμre issμed a notice of violation"severely crack down on the illegal prodμction and operation orμse of protein powder", explicitly strictly prohibited in feed melamine, adhere to the zero detection of melamine in feed. All the acts are criminal of artificial addition of melamine in the feed ,shoμld be resolμtely foμght against.
Keywords/Search Tags:Melamine, Residue, HPLC, Chicken
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