Research Of The Activity Of Embryo Protein Extracted From Chicken On HepG₂ Cell Apoptosis Cultured In Vitro

Experiments proved that animal embryo and its extract can affect the direction of tumor cell differentiation. The development of embryo has been accompanied by the differentiation of cell and the apoptosis of cell, the reason of tumor development is implant apoptosis. As the embryo grows, its differentiation factor not only catalyzes embryo cell differentiation and apoptosis, but also introduces the embryo growing in a certain direction. So the application of this differentiation factor in the catalysis of tumor cell differentiation and apoptosis draws much attention in recent years.Salt-soluble means that the solubility of protein grows while the salt concentration increases in low salt concentration. When salt concentration keeps increasing, the solubility of protein will decrease to some extent, and then the protein separates out. Separation and purification can be achieved through the latter salting process. As the most important means of separating protein, salting is commonly implemented by neutral salt, for example, sodium sulfate, sodium chloride, sodium phosphate and ammonium sulfate.This experiment took chicken embryos in different growing stages as material. Due to the stability and high solubility, ammonium sulfate method was adopted to extract protein from Chicken embryos extracts. Single factor was used to optimize the extracting conditions of extraction temperature, extract concentration, solution pH, and ammonium sulfate saturation. The results showed that the optimum conditions of protein extraction from Chicken embryos extracts were extraction temperature 4℃; extract concentration 25%; solution pH 6.6; and ammonium sulfate saturation 60%.In the optimized conditions, this experiment extracted proteins from chicken embryos of different developmental periods, dialyzing with 8000-140000 dialysis bags for 24-36 h, and then using HELP to further separate the proteins. Proteins in different shape of peak were collected, freezed, and dissolved in a certain volume of DMEM, and finally, were used for the induction of tumor cells in vitro. The results showed that the higher the molecular weight, the more obvious induction effected. In addition, we found that the proteins in the early developmental embryos for liver cancer cells induced more obvious than the proteins in late embryos by a series of cell apoptosis testing.