The Extraction And Purification Of Alkaloids From Rhizoma Coptidis And Their Pharmacokinetics In Mice

This article selects material Shizhu the Chongqing Rhizoma Coptidis by-product fibrous root of Rhizoma Coptidis to have。The earlier period research indicated the fibrous root of Rhizoma Coptidis the total alkaloid contents to achieve the Rhizoma Coptidis 1/3, but the market survey demonstrated that the fibrous root of Rhizoma Coptidis price each kilogram be the Rhizoma Coptidis 1/15。The alkaloid pharmacological action is more and more widespread along with the Rhizoma Coptidis in is paid attention, especially existing very many drop the blood sugar about the Rhizoma Coptidis alkaloid, drop the blood fats, drop research and so on blood pressure, anti-tumor. Therefore, the development the beard of Rhizoma Coptidis the cost be low, the selection is convenient, the medicine value is big, the prospect is broad。This article studied the Chinese goldthread rhizome to have to hit the alkaloid the extraction condition and the craft; Then the beard of Rhizoma Coptidis extract the separation purification experiment with each kind of resin to alkaloid; Eventually has studied the Chinese goldthread rhizome alkaloid in mouse in vivo metabolism and a medicine generation of dynamics。The concrete result is as follows:1. Rhizoma Coptidis alkaloid extraction craft optimizationHad the Rhizoma Coptidis to the laboratory to have the root each extraction method to carry on the confirmation through the experiment, selects the cost lowly, the effect good extraction method, and carries on the optimization to the extraction condition。Finally determined the beard of Rhizoma Coptidis the total alkaloid extraction condition be 0.3% sulfuric acid water immersion soaks, heating backflow extraction。2. The Chinese goldthread rhizome whisker extraction and separation experimentThe the beard of Rhizoma Coptidis extract separation experiment:Selects the different resin and the absorbent to extracts carries on the adsorption experiment, finally determined the low-acid positive ion resin D152 adsorption effect is good, the adsorptive capacity is big。The optimized adsorption parameter, will extract with the milk of lime pH neutral 5-6, the adsorption effect will be best。3. Rhizoma Coptidis alkaloid purification experimentRhizoma Coptidis alkaloid purification experiment:To adsorbs on the D152 resin column the Rhizoma Coptidis total alkaloid elution, the collection, the examination With certain density NaOH solution elution resin column, can elute the medicine root alkali monomer; Can elute the surplus complete alkaloid with the certain sulfuric acid density methyl alcohol (70%) solution。Carries on the salting out with different density NaCl, can obtain berberine monomer and the total alkaloid。4. The metabolism experiment of the Rhizoma Coptidis alkaloidSelects many kinds of chemical additives and the total Rhizoma Coptidis alkaloid mix disposes the experimental medicine, carries on metabolism experiment。Can through fill the stomach to the mouse, in the examination mouse excrement rhizoma coptidis total alkaloid remaining quantity how many, screens the biggest promotion rhizoma coptidis total alkaloid absorbing capacity to help the medicinal preparation; The data uses statistics processing method。5. Rhizoma Coptidis alkaloid medicine generation of dynamics researchWill test selects helps the medicinal preparation to join the rhizoma coptidis total alkaloid superiorly, will fill the stomach to the mouse, will draw blood through various time section to the mouse eye socket, will examine the mouse blood medicine density with HPLC, thus understanding medicine in mouse in vivo medicine generation of dynamics parameter。...