The Experimental Study On Functioning Mechanicsm Of MMP-13 And IGF-Ⅰ In The Synovium Of Osteoarthritis And Kashin-Beck Disease

Objective To understand the role of MMP-13 (matrix metalloproteinases) and IGF-1 (insulin-like growth factor) in pathological mechanism of synovium from knee osteoarthritis (OA) and Kaschin-Beck disease (KBD) and compare their similarities and differences.Methods To extract joint synovial fluid in 18 cases of OA patients and 13 cases of KBD patients and six cases of the normal ,Then to compare the ratio of positive cells of MMP-13 and IGF-1 in synovial tissue and through the sandwich Elisa to detect protein content of MMP-13 and IGF-1 in Synovial fluid in the joint.Results In this group of 37 samples of synovial tissue and synovial fluid : 1) the ratio of MMP-13-positive cells in synovium in KBD patients with the ratio of OA patients showed no significant difference (P> 0.05), with the control group Comparison is a significant difference (P <0.05). 2)The ratio of IGF-1 protein expression in synovium in KBD patients with the OA group and the control group were significantly different(P <0.05). 3) In synovial fluid of KBD patients the content of MMP-13 with the colonial OA group were no significant difference P> 0.05), compared with the normal control group there were significant differences (P <0.05). 4) The content levels of IGF-1 in synovial fluid of KBD patients with OA group and the control group were significantly different.Conclusion. 1) Like OA, the synovial lesions in KBD patients were existed as a possible link with degeneration of cartilage and destruction of joint. 2) As the main factors of decomposition in pathogenesis of OA ECM(cartilage extracellular matrix),MMP-13 may be involved in the evolution of pathology of synovium in the OA and KBD patients . 3) As a factor of ECM, IGF-1 also participated in synovial pathological process from the OA and KBD patients, but the repair mechanism in ECM from the two diseases may be different.