Study Of Effect Of Flavonoids From Pteridium Aquilinum On Cervical Cancer And Its Mechanism

Cervical cancer is a malignant tumor in genital system that threatened the health and the life of women. The etiological factor of cervical cancer is not clear yet, and it might be related with many influencing factors. Because of the multiformity of the reason and the mechanism behind the cervical cancer, it is very difficult to prevention and to treatment. Furthermore, because of the side-effects and the multi-drug resistance in treatment (e.g. chemotherapy), screening of anti-cervical cancer drug especially plant source anti-cervical cancer drug becomes the most important task of tumor pathology and toxicology research. There is no report on the effects about the anti-cervical cancer research in multiple-angle, and in this study, the cervical cancer-bearing mice models and Hela cell line was set up in vitro and in vivo to study the effects of the anti-cervical cancer of flavonoids from pteridium aquilinum.Pteridium aquilinum is a drug-food plant, and has a health-care function. Being a drug, pteridium aquilinum has functions of fever reducing, intestine smoothing, phlegm dispersing, anchoring mind and antihypertensive effects and so on. But there is no report about whether the flavonoids from pteridium aquilinum have anti-tumor effects, so in this paper, we studied the inhibition effects of the flavonoids on the mice U14 tumor cells in vivo and human Hela cell line in vitro, and the modulating effects of them on the immune system in mice models.Flavonoids from pteridium aquilinum was got by the method of ethanol extraction, and further purified by solvent extraction. After the flavonoids from pteridium aquilinum were got, we studied the effects of them on the transplanted cervical-cancer in mice. The results showed that Flavonoids from pteridium aquilinum could inhibit the growth of solid tumor in mice, the inhibition rates of low, middle and high doses could reach 38.79%,44.54% and 54.02%, respectively. And this extract could also prolong the life span of ascites tumor-bearing mice, the life span increasing rates reached 49.39%, 62.20% and 73.00%. Furthermore, Flavonoids from pteridium aquilinum exhibited strong toxic effect on Hela cell line in vitro, and the inhibition rate was 89.3%. Also, we found that all of the doses of flavonoids from pteridium aquilinum could increase the indexes of thymus and spleen in tumor-bearing mice, which suggested that this extract could promote the growth of immune organs.The slices of liver and kidney in flavonoids from pteridium aquilinum treatment mice showed that the cells in liver and kidney were lined up compactly and regularity, and there is no pathology changes in the two important organ, which indicated that there was no obvious toxic effects on liver and kidney in tumor-bearing mice.Flavonoids from pteridium aquilinum treatment could improve the T-SOD and T-AOC levels in tumor-bearing serum significantly, but down-regulated the MDA level, and up-regulated the T-AOC, T-SOD and GR levels in thymus and spleen tissues of tumor-bearing mice.The results of immunohistochemistry showed that flavonoids from pteridium aquilinum could inhibit the expression of Bcl-2 and mutant p53 genes, improve the expression of Bax gene.Flow cytometry results showed that most Hela cells was inhibited in G1 phase by the treatment of flavonoids from pteridium aquilinum and the cells in S and G2/M phase were decreased significantly, and the cell proliferation was inhibited further lead to the apoptosis of tumor cells.In this paper, we preliminarily revealled the function mechanism behind the effects of tumor inhibition and immune capability improvement by the experiments in vitro and in vivo, and all of these results will provide theoretical basis for further developing flavonoids from pteridium aquilinum as an natural anti-tumor drug.