Molecular Epidemiology Study Of Hepatitis B Virus Infection In Swines

Objectives:To detect the hepatitis B virus infection in swines,to make sure whether the hepatitis B virus exist in swine or not, for understanding the prevalence of swine hepatitis B virus infection, discussing the feasibility of that swine is used for animal models of hepatitis B virus infection.Methods:283 swine blood was collected in two tubes,one was used to get serum by centrifuging,and get 234 serums;the other was used to extract white blood cell(WBC) DNA,and get 156 DNA.Serum HBV maker(HBV-M) were detected by enzyme-linked immunosorbent assay(ELISA),and HBV-DNA of the serum samples and white blood cell were tested by nested polymerase chain reaction(PCR) with two pairs specific primers. A patient who infected in HBV.and serum primary marker (HBsAg+,HBeAg-,HBcAb+)was positive. Whose serum was used for posititve control.Results:(1) 234 serum samples were detected HBV M:there are 8 models of HBV M positive:model 1 (HBsAg+,HBeAg+,HBcAb+), model 2 (HBsAg+,HBeAb+. HBcAb+),model 3 (HBsAg+,HBcAb+),model 4 (HBsAb+,HBeAb+and HBcAb+),model 5 (HBsAb+and HBcAb+),model 6 (HBsAb+),model 7 (HBeAb+and HBcAb+), model 8 (HBcAb+).and their rate are 0.43%(1/234),0.85%(2/234),2.99%(7/234),0.43%(1/234), 26.07%(61/234),2.99%(7/234),15.38%(36/234).34.19%(80/234),respectively.(2) The PCR results of serum HBV-DNA:8 serums were detected with S primer, and 47 serums were detected with C primer, the positive rates were 3.4%(8/234),20%(47/234),respectively;(3) The PCR results of white blood cell HBV-DNA:17 were detected with S primer, and 80 were detected with C primer, the positive rate were respectively 10.9%(17/156),51.3%(80/156);(4) The positive rates of white blood cell HBV-DNA is higher than serum HBV-DNA, and the difference is statistically significant(p<0.001);(5) the positive rates with C primer is higher than the positive rates with S primer, and the difference is statistically significant(P<0.001).Conclusion:(1)There are higher prevalence rate of swine HBV in swine, and the infection mode is similar to human infection mode;(2)The positive rates of white blood cell HBV-DNA is higher than serum;(3)The test with C primer was more sensitive than test with S primer.