Polymorphism Of 17 Y-chromosomal Short Tandem Repeats Loci In 10 Minority Populations In Guangxi Of China

Y chromatosome is sex-chromosome, it is presence on normal male .when the Y-chromosome is inheritance from father to son, the sequence of gene is Constant excluded the pseudoautosomal region unless variation is occurrence.Therefore,a men can be appreciationed which nation he is through the test of Y-chromosome short tandem repeats(STR) . Also the test of Y-STR is powerful for Judging Paternity and genealogy. In the mixed Stains from Sexual Assault Cases, the tested Y-chromosome STRs is better than the tested autosomal STRs.For researching the Polymorphism of Y-STR loci in the minority populations in Guangxi of China, we investigated the genic information of 17 Y-STR in don,miao,yi ,gelao, melao, maonan, jing,yao,shui and zhuang nationality of Guangxi of China,and then evaluate their application in forensic and genetics.as well as the information of polymorphism is significant to build DNA database.The oral swab came from1149 independence male of guangxi in china.dong is 101,miao is 101,zhuang is 107,yao is105,shui is 150,gelao is 130,melao is 146,jing is 100,yi is101,maonan is 108. the DNA was extracted from oral swab of minority population with magnetic beads method and then seventeen Y-STR loci were amplified with AmpFlSTR? Yfiler^TM PCR Amplification Kit System. The PCR products were detected with 3l30XL Genetic Analyzer.The allele frequencies was calculate directly,diversities and genetic distance were calculated by the equation: h=n(1-∑Pi²)/(n-1).TGD was calculated by the equation:TGD=1-(1-GD₁)(1-GD₂)(1-GD₃)(1-GD₄)…(1-GD_n). Nei'DA distance was calcu- lated by DISPAN soft. compared the allele distribution between 10 minority populations and han nationality by arlequin 3.11soft.and AMOVA base on allele frequencie is performed by arlequin. N-J tree base on DA distance was draw by MEGA 4.0 soft. MDS base on DA distance was performed by SPSS 12.0.The information of allele distribution and gene diversity of the 17 Y-STR loci were obtained from ten minority population. 96 allele exclude DYS385a/b and 66 haplotype of DYS385a/b was find , The allele frequencies is 0.0066-0.9307 .1129 haplotypes of 17 Y-STR were found in 10 minority population, only one haplotype are shared between gelao and miao population. One haplotype is twice in dong,jing, maonan population. two haplotype is twice in zhuang,miao,gelao population. three haplotype is twice in shui,mielao population. four haplotype is twice in yi.GD of the 17 Y-STR loci is 0.1327-0.9663 in ten minority population. HD is 0.99920-0.99983.TGD all is close to 1.AMOVA indicate that genetic variance between ten population is major,it is 7.23%. D_A distance, N-J tree and MDS indicate that there have significant difference between white people and yellow people.The significant difference is presence between han nation and ten minority population, also interior of ten minority population.Conclusion : Single Y-STRs is less capability of discrimination than the multiple-Y-STRs. From ours research,we know that :1.the haplotype diversity of 17 Y-STR loci is so effective in identity discrimination , paternity identification and test of mixing stains. 2.To estimate which nation or racial a man is ,the haplotype diversity of 17 Y-STR have significance. 3. haplotype diversity of 17 Y-STR is one of significant technique to research history of nation or migration of human population. also, It is significant to build DNA database of minority population.