Study Of Antimicrobial Activities Of Compounds Isolated From Traditional Chinese Medicines And Anti-inflammatory Activities Of Myricetin

Pathogens infect the host and the proliferation induces acute and chronic inflammation. Inflammation closely linked to the occurrence and development a variety of major diseases. Many traditional Chinese herbal medicines are with anti-bacterial anti-inflammatory functions. Moreover, a growing number of herbal anti-bacterial anti-inflammatory constituents and their antimicrobial and anti-inflammatory mechanism been elucidated. However, the inhibition of bacterial growth and inhibit inflammation are two different processes. What are active ingredients in the Chinese herbal medicines? Can they suppress the growth of bacteria? Whether these components have a direct anti-inflammatory effect? The study of these issues to clarify the mechanism of anti-inflammatory anti-bacterial medicine is essential. This paper attempts to explore these issues and problems.This paper studied the traditional Chinese medicine monomer gallic acid lactone, 3,4,5 - trimethoxy benzoic acid, baicalin, puerarin, myricetin, shikimic acid, pyrogallic acid of several common pathogens antibacterial action of these compounds were determined by the minimum inhibitory concentration of bacteria. Found that isolated from traditional Chinese medicine compound gallic acid lactone, myricetin, pyrogallic acid with a good role in inhibiting bacterial growth. On this basis, the selected TCM monomers myricetin were inflammatory cells and animal experiments.Myricetin (3, 3', 4', 5, 5', 7-hexahydroxyflavone), a natural flavonoid commonly found in is a tea, wines, fruits and many plants, has been shown to exhibit antioxidation, anti-inflammatory, antimicrobial and anticarcinogenic activities. However, the anti-inflammatory mechanism of myricetin and the effects of myricetin on proinflammatory cytokines release are not well defined. Here we examine the inhibitory activity of myricetin on a number of matrix metalloproteinases, evaluate its protective effect on oxidative cell damage, and used animal model to monitor the its effect to treat inflammation and on proinflammatory cytokines releases. We found myricetin showed strong inhibition to matrix metalloproteinase-1, -2, -9, -14 and -16 enzymatic activities in vitro. Pretreatment of myricetin effectively relieved H2O2 induced cell damage. In vivo, myricetin reduced the release of TNF- and IL-12 induced by LPS. Moreover, mice with acute inflammation induced by carrageen was suppressed though myricetin treatment. Overall, our data suggest that myricetin can attunate inflammation at least though its MMP inhibition and antioxidative activities.In this paper we first found several of common pathogens are subjected to the inhibition of traditional Chinese medicine compounds, revealing the anti-inflammatory medicine monomer myricetin effect and molecular mechanism. The results show that some traditional Chinese medicine compounds, such as myricetin can simultaneously inhibit the growth of pathogenic bacteria and to inhibit the inflammation process. Our study has made a certain contribution to the clarification anti-bacterial anti-inflammatory mechanism of Chinese medicines.