Inhibitory Effects Of 1,8-cineol On Ovalbumin-induced Lung Inflammation And Airway Hyperresponsiveness In A Guinea Pigs Model Of Asthma And The Regulation Of Th1/Th2 Balance In A Mice Model Of Asthma

AIM To investigate the antiinflammatory activity of 1,8-cineol in a guinea pigs model of ovalbumin-induced asthma.METHODS The guinea pigs's model were performed by intraperitoneal injection of the gel including 20μg OVA and 20 mg Aluminium Hydroxide.The Guinea Pigs were constructed by immunization of intraperitoneal injection at date 0 and date 7,and the experiment were performed after 28 d.The effect of 1,8-cineol 10,30 and 100 ml·kg-1 on the Raw and Cdyn of asthmatic guinea pigs after OVA challenged(concentration is 1%) for 1 h.The changes of leukocyte and different kind of leukocyte after the challenge of OVA has been studied;The levels of ECP,IL-4,IL-8 and TNF-αin lung of guinea pigs were determined using enzyme-linked immunosorbent assay(ELISA).The changes of%Raw and%Cdyn of asthmatic guinea pigs were investigated after OVA challeged 17 h.The changes of leukocyte and different kind of leukocyte after the challenge of OVA has been studied;The levels of ECP,IL-4,IL-8 and TNF-αin lung of guinea pigs were determined.Using enzyme-linked immunosorbent assay(ELISA).RESULTS 1,8-cineol inhibited the increase of Raw and the decrease of Cdyn from 1 to 30 min after OVA challenged in the model of guinea pigs(P＜0.01);The levels of ECP,IL-4 and TNF-αof asthmatic model group [(2.53±2.24)μg·L-1,(1.85±1.01)ng·L-1 and(1.534±0.91)ng·L-1]were higher than those of normal control group[(0.46±0.02)μg·L-1,(0.90±0.02) ng·L-1,(0.69±0.02) ng·L-1](P＜0.05).The levels of ECP,IL-4 and TNF-αof 1,8-cineol 100 mg·kg-1 group [(0.51±0.03)μg·L-1,(0.90±0.02)and(0.69±0.03)ng·L-1]were significantly lower than those of asthmatic model group(P＜0.01).The level of IL-8 of asthmatic model group didn't have any significant difference from that of control group(P＞0.05).1,8-cineol 100 mg·kg-1can significantly decrease the numbers of leukocyte and the percent of eosinophils in BALF;It have significate difference from control group(P＜0.05);After 17 hous from ova challenge,%Raw of asthmatic group[(89.24±20.5)%]is higher than control group[(28.3±6.82)%](P＜0.05);%Cdyn of asthmatic group[(-52.6±22.2)%]have the significant difference from control group[(-15.7±2.46)%](P＜0.01);1,8-cineol 100 mg-kg-1 inhibited the increase of%Raw[(41.4±7.16)%],which have the significant differnce from model group(P＜0.05);1,8-cineol 10,30and100 mg·kg-1 improve the decrease of%Cdyn after 30 mg·ml -1 Mch-induced in the model of guinea pigs which were challenged by OVA after 17 hours[(-28.1±3.1)%,(-17.6±2.04)%,(-18.6±3.44)%]; 1,8-cineol100 mg·kg-1can significantly decrease the numbers of leukcyte and the percent of neurophils,the levers of ECP,IL-8,TNF-α[(4.11±0.13)μg·L-1,(5.38±0.58)and(4.38±0.18)ng·L-1]of asthmatic guinea pigs significantly higher than those of control group[(3.57±0.05)μg·L-1,(4.64±0.70) and(3.73±0.11)ng·L-1](P＜0.05); The levels of ECP,IL-8 and TNF-αof 1,8-cineol 100 mg·kg-1 group[(3.26±0.13)μg·L-1,(4.65±0.18)and(3.66±0.04)ng·L-1]were significantly lower than those of asthmatic model group(P＜0.01).The level of IL-4 of asthmatic model group didn't have any significant difference from that of control group(P＞0.05).The OD number of IL-4, IL-5,Eotaxin in the model group of asthma mice is higher than the control group (4.02±1.23,1.75±0.39,4.24±2.94,P＜0.05).The OD number of IFN-γin the model group of asthma mice is significantly lower than the control group.(P＜0.05).Prednisone and 1,8-cineol 100 mg·kg-1can decrese the OD number of IL-4,IL-5,Eotaxin(P＜0.05). CONCLUSION These results suggest the possibility that 1,8-cineol can exert suppressive effects on asthma and may provide evidence that 1,8-cineol is a useful agent for the treatment of allergic airway disease and can modulate the balance of Th1/Th2.