| Objective:Through used prostaglandin E1 in the rat model of ischemia/reperfusion injury, we investigate the protective effects of PGE1 on small intestinal ischemia/reperfusion injury by analyzing the small intestinal mucous membrane's extent of damage,the inflammatory cell factor's release and bacterial translocation, to provide a new strategy for clinical to prevent from small intestine ischemia/reperfusion injury.Methods:Eighty male SD rats were weighed about 280-320g, then they were divided randomly into four groups:Sham group(S group), ischemia/reperfusion injury group(IRI group), ischemia preconditioning group(IPC group), Prostaglandin El group(PGE1 group)(n=20).Each group was divided into two subgroups according to time of specimen collection after reperfusion(n=10).Before 12 hours of testing, the testing rats were gaven fasting and free drinking; and before 2 hour of testing, the testing rats were irrigated colon bacillus (E.cdi DH5a)which contained green fluorescent protein into stomach.①The S group:the rats underwent a 3cm medium length-wise laparotomy after anesthesia, identification and dissection of the superior mesenteric artery(SMA)and then, closed abdominal cavity.②The IRI group:identification and dissection of the SMA as S group, the SMA was clipped by a un-wound micro artery clip, and then, closed abdominal cavity; after 45min,taken out the un-wound micro artery clip; then collected specimen at the time of 2h and 4h. ③The IPC group:identification and dissection of the SMA as S group,the SMA was subjected to 3 circles of 2min ischemia and 2min reperfusion before ischemia, and then, same as IRI group.④The PGE1:group identification and dissection of the SMA as S group, t the SMA was clipped for 45 min,5 min of reperfusion,inject PGE1 from tail vein(20ug/kg), and then, same as IRI group.At the end of the before operation, we dissected 1cm segment of ileum to determination of intestines mucous membrane's injury. Then we measured the serum of TNF-a, IL-1β, IL-6, IL-10 by ELISA. The last,the rats were sacrificed, we cut tissues of liver,spleen at the same weight(2g) and portal vein blood to train bacterial translocation。Results:①The changes of intestinal mucosa:In Sham group, under microscope, the intestinal villus were completely and linedly;In IRI group, the intestinal mucosa presented dilated and exposed capillaries and denuded villi, some of villi hemorrhage were ulceration;the lamina propria was edema and infiltrated with inflammatory cells. The intestinal mucosal structure maintain intact with lifting of the epithelial layer from the lamina propria and moderate extension of the subepithelial space in PGE1 group and in IPC group. In PGE1 group and IPC group, histopathologic scores were significantly lower than that of IPI group(P<0.05). There were no significant difference between PGE1 group and IPC group (P>0.05).②Compared with S group, the serum contents of TNF-a,IL-1β,IL-6 in IRI group, IPC group and PGE1 group were obviously higher, but the PGE1 group and IPC were lower than that of IRI group. The contents of IL-10 in other three groups were lower than that of S group, and The PGE1 group and IPC group were higher than that of IRI group. There were no significant differences between PGE1 group and IPC group (P>0.05).③acterial translocation:In S group, the bacteria wasn't checked out from portal vein blood,liver tissues and spleen tissues at 2h and 4h. But PGE1 group and IPC were lower than that of IRI group (P>0.05).There were no significant differences between PGE1 group and IPC group (P>0.05). Conclusion:The PGE1 and IPC make rats small intestinal mucosa's extent of damage alleviated after reperfusion, can protect small intestinal mucosal barrier; can obviously inhibit the release of TNF-a,IL-1β,IL-6 and promote anti-inflammatory medium of IL-10 expression after reperfusion;can obviously inhibit the bacteria transposition after reperfusion。...
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