The Expression And Significance Of Caspase-8,cFLIP In Colorectal Neoplasms

Objective: To explore the effect and significance of Fas- associated death domain-like interleukin-1 converting enzyme (FLICE)- like inhibitory protein (cFLIP) and cystine asparate specific protease-8 (caspase-8) in diagnosing and treating with colorectal neoplasms, we investigated the expression of in colorectal neoplasms at mRNA level, and analyzed relationship between the occurrence and development of colorectal neoplasms with them,Methods: The expression of cFLIP,caspase-8 was detected in colorectal neoplasms, nearby tissues and matched normal tissues, at mRNA level, was detected by SYBR Green I quantitatie realtime polymerase chain reaction technique.Result: The relative expression amount of cFLIP, caspase-8 in colorectal neoplasms tissues were (1.913±0.845) , (1.856±0.810) , respectively; The relative expression amount of cFLIP, caspase-8 in nearby tissues was (1.521±0.447), (1.597±0.578) , respectively. The expression of them in neoplasms and nearby tissues was higher than that in normal tissues(p < 0.05). No relationship could be exist in cFLIP and clinicopathological parameters (including sex, age, differentiation degrees, size, lymph node metastasis, distant metastasis, invasion, distribution) (P>0.05), while caspase-8 in group with lymph node metastasis seemed much higher than that in group without lymph node metastasis (P<0.05), and there were no statistical differencese among groups devided by other different clinicopathological parameters (P>0.05).Conclusion: The overexpresstion of cFLIP and caspase-8 played a great role in the occurrence and development of colorectal neoplasms, but there might be no linear relationship between them. We couldn't observe cFLIP correlated with clinicopathological parameters, while caspase-8 in group with lymph node metastasis was higher than that in group without lymph node metastasis. It suggested that caspase-8 had close relationship with the malignant degree and prognosis of colorectal neoplasms. To sum up, we concluded cFLIP and caspase-8 had close relationship with the occurrence, development and prognosis of colorectal neoplasms, and them could be new targets of the diagnosis and treatment of colorectal tumors in the future, hopefully.