The System Evaluation And Clinical Significance Of Immunological Function For The Advanced Lung Cancer Patients

objectiveThe actual evaluation of immunological function is significant for studingthe tumor development and devising a treatment in time. This study was aimed to evaluate the immunological function of advanced lung cancer patients systematically, and to discuss the clinical significance.MethodsThe nucleated cell amounts of advanced lung cancer patients and the healthy individuals was counted. The immune cell subsets and the levels of IL-4, IFN-r, perforin, granzyme and immune suppressor cell(Treg) with the flow cytometry were measured. The inhibition ratio of immune cells to several tumor cell lines was evaluated by MTT assay and their proliferation activity.ResultsThe absolute amounts and subsets of T, B, NK cells of advanced lung cancer patients was lower than the healthy individuals(P<0.05); However, the proportion of regulatory T cells of advanced lung cancer patients (4.00±1.84)%was lower than the healthy individuals (1.27±0.78)%, P<0.05. The positive rates of IFN-r perforin, granzyme in CD8+T cells decreased while them in IL-4 did not in the advanced lung cancer patients compared to the healthy control group (P<0.05). The proliferation activity of immune cells, the positive rate of PPD masculine and the inhibition ratio to tumor cells in the advanced lung cancer patients was lower than the healthy subsets obviously (P<0.05). ConclusionThere was a significant immune depression and immunosuppressive action enhancment with the amounts,subsets,killing activity in vitro,proliferation ability,PPD response in vivo,cytokine of immune cells and immune suppressor cells in the advanced lung cancer patients compared to the healthy individuals.It can be theoretical foundation for clinical therapy.