Age-related Changes And The Correlation Of MDA And AGEs Content Of The Serum And Saliva In Healthy Adults

Anti-aging in China has become a hot issue since it stepped into an aging society. Aging can be divided into two categories:pathological aging and physiological aging. Pathological aging is the organ degeneration caused by a variety of chronic diseases; whilst physiological aging is the degenerative changes of the organism occurring gradually over time due to the decline in metabolic function. The individual physiological aging can be reflected by the biological markers as the premise of a quantitative analysis. The current studies are mainly on cell and molecular level and the theories is mainly oxidative stress theory of aging and non-enzymatic glycation theory of aging. Previous study was to determine the relationship between MDA/AGEs of blood and tissue and aging (MDA is a product of lipid peroxidation, AGEs is the end products of non-enzymatic glycation). Compared with blood testing, saliva testing is non-invasive, easily sampled and accepted by patients. The age-related changes and the correlation of MDA and AGEs content of the saliva in healthy adults have not been reported. Whether could test the body's aging level through the determination of their content of saliva? Therefore, the aim of this study is to investigate the age-related changes of MDA and AGEs content of the serum and saliva in healthy adults to explore whether saliva testing is served as measured biological indicators of aging instead of blood testing.Objective:To explore whether saliva testing can be served as an alternative to blood testing in the measurement of biological indicators of aging by investigating the age-related changes of MDA and AGEs content of the serum and saliva in healthy adults, and to provide the evidence for further studying the biological markers of aging in saliva.Methods:A hundred and twenty healthy adults were divided into 4 groups by age: Group 1 (20-39 years), Group 2 (40-59), Group 3 (60-79) and Group 4 over 80 years, respectively,30 in each group. The MDA content of the saliva and the serum in healthy adults were determined by using MDA kit. The AGEs content of the serum and the saliva in healthy adults were determined by using human advanced glycation end products ELISA kit.Results:1. The MDA content of the serum and saliva(1) The MDA content of the serum and saliva in 60-79 years group and over 80 years group were significantly higher than that in 20-39 years group and 40-59 years group.(p< 0.05). The MDA contents of the serum and saliva in 60-79 years group and over 80 years group were not significantly different in the healthy adults (p> 0.05). The MDA contents of the serum and saliva in 40-59 years group and 20-39 years group were not significantly different in the healthy adults (p> 0.05). (2) There is distinguished right dependability in serum and saliva (r= 0.79, p< 0.01).2. The AGEs contents of the saliva and serum(1) The AGEs contents of the saliva and serum in over 80 years group was the highest of all groups in the healthy adults.(p< 0.05).40-59 years group and 60-79 years group were significantly higher than that in 20-39 years group (p< 0. 05).40-59 years group were higher than that in 20-39 years groups.(p< 0.05). 40-59 years group and 60-79 years groups were not significantly different (p>0. 05); (2) There is distinguished right dependability in serum and saliva (r=0.90, p <0.01).Conclusion:(1) The AGEs and MDA contents of the serum and the saliva of healthy adults decrease with aging.(2) The detection of AGEs and MDA contents in the saliva can serve as an alternative to that of the serum as a biological sign of senescence.