Study On The Chemical Constituent Of Crude Saponins From Roots Of Radix Phytolaccae And Its Criterion Revision In Chinese Pharmacopoeia

Radix Phytolaccae(Chinese name shanglu),a famous Chinese herbal medicine collected in Chinese pharmacopoeia,is derived from the root of Phytolacca acinosa Roxb.or P.americana L.. It has been long used for treatment of edema,abdominal fullness,dysuria,abnormal defecation,and external application for the treatment of carbuncles and sores as a traditional Chinese medicine(TCM).The major constituents of R. Phytolaccae are PEPs,protein and triterpene saponins. The major biological activity of the compounds in R. Phytolaccae is attributed to its triterpene saponins, which have been reported to have a variety of activities such as induction of immune interferon(INF-c),anti-inflammatory agents,and tumor necrosis factor(TNF);enhancement of leukocyte phagocytosis and promotion of DNA transformation;antifertility;and molluscicidal,etc.For supplying a more convenient method to get these saponins,we did lots of relative researches as following aspects:Firstly we summarized the researches on R. Phytollaccae in recent 30 years,including the plant,preparing,constituents,content determine,bio-activities and toxicity.The second part is the separation and purification of crude saponins from the roots of R. Phytolaccae:(1)By using several chromatographic methods,we got 7 compounds:daucosterol,esculentoside B, esculentoside D, esculentoside C, esculentoside T, esculentoside A(EsA) and esculentoside H, esculentoside T is a new compound. (2)In order to get large number of EsA quickly,the procedure of separation and purification of EsA was also investigated.As a CRS in pharmacopoeia, EsA was added a criterion by us. (3)We established a method for the purification of four saponins such as esculentosides A-D from a crude extract of R. Phytolaccae by HSCCC coupled with an evaporative light scattering detector.Meanwhile we assumed one standard research Project of Pharmacopoeia of People's Republic of China Edition 2010:Quality specification revision of R. Phytolaccae.A special HPLC-ELSD method was adopted for the determination of EsA in medical material and processed product of R.Phytolaccae from different places,then a content limitation was established.We described the shape and properties of R. Phytolaccae and established a TLC method in order to distinguish the medical material.Lastly the limitations of water content,acid-insoluble ash content and extractives of R. Phytolaccae(processed with vinegar) were established.On the base of the studies above,the quality specification of R. Phytolaccae was successfully revised.