| Rhubarb is a commonly used clinical medicine. It has a history of the millennium for treatment. In China there are 45 varieties and 2 varieties, it's mainly distributed in Gansu, Qinghai, Sichuan, Shaanxi and Tibet. It is the dried rhizome of Rheum palmatum L., Rheum tanguticum Maxim.ex Balf. and Rheum officinale Baill. according to Pharmacopoeia of the People's Republic of China (2005 edition).Rhubarb is traditionally used for purging heat, cooling blood, detoxification, break blood and expel stasis. In addition, Rhubarb also can be used to broad-spectrum antisepticize, protective and choleretic, anti-tumor, drop blood fat, etc. It has a wide range of pharmacological effects with strong foundation and tremendous medicinal potential.The recent comprehensive quality control of rhubarb is more focused on only one ingredient or one kind of ingredients by TLC and HPLC. However, the pharmacological effect of rhubarb is played by a class or a certain kind of pharmacological activity components. The above methods have showed some limitations in comprehensive quality control of rhubarb. Thus, a valid, simple and comprehensive quality control method is need to be established.29 batches of Rhubarb were determined with 5 pharmacologically active ingredients and done the fingerprint analysis in the basis of both quantitative and qualitative analysis by HPLC. The good results were obtained.Firstly, HPLC method was established for quantitatively simultaneous determining Gallic acid, Sennosides A, Aloe emodin, Emodin and Chrysophanol in Rhubarb. The method is stable and reliable, good reproducibility. In which Sennoside A is the strongest purgative component, followed by anthraquinone; Emodin and Aloe emodin has the strongest antibacterial activity; Gallic acid and Chrysophanol play the major role of hemostasis. So the simultaneous determination of the 5 kinds of basic compounds can consummated the Quality Control of Rhubarb from the view of pharmacological. Through testing and screening, the preparation method of medicinal products was selected. Only methanol extracted, without too much action. It was fast, accurate and nondestructive.Secondly, the HPLC fingerprint of Rhubarb was established. Different polarity ingredients were separated and retained the right time by the method. The run time is 75min and about 50 peaks were obtained.29 batches of Rhubarb were determined,30 distinguished peaks were found. The fingerprint was divided into 4 zones in order to do the identification of Rhubarb. After comparing the fingerprints, there were some differences between the 29 batches of Rhubarb. Because Rhubarb is from multi-source, fingerprint should be established for different varieties.This paper was focused on quality control of Rhubarb, through the research on chemical determination and HPLC fingerprint by choosing quality control conditions and steps. We try to do more research on quantitative chemical composition and qualitative content types of Rhubarb from the pharmacological point of view and lay a foundation for the establishment of a comprehensive, integrated system of quality control of Rhubarb.
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