The Observation Of Effects Of Cnidium Monnieri Cusson In Experimental Osteoporosis Rats

Objective:To observe the therapy effect of Cnidium monnier Cusson on osteoporosis of ovariectomized rats. And investigate the association between Cnidium monnier Cusson and IGF-1 and vitamin D.Methods 40 female 10-month-old SD rats were randomized into 4 groups:Sham group, OVX group, CT group, Raloxifene hydrochloride group. The experimental model of osteoporosis was established by removing twin ovary operation. Given Cnidium monnier Cusson 12mg/kg body weight P.O. for 8 weeks, Bone mineral densities of global and the lumber spine and femur neck were measured with Hologic dual energy x-ray bone densitometer in rat. Femur trabecular area, bone mineral deposition were measured after the end of therapy. Serum IGF-1 levels and serum 25OHVitD concentrations and lymphocyte Vitamin D receptor contents were determined by means of ELISA.Results BMD of the lumber spine and femur neck in Cnidium monnier group and Raloxifene group were higher than that in OVX control group (P<0.01). Cnidium monnier increased trabecular area, bone deposition compared with OVX group. The serum IGF-1 and 25-OHVitD concentration in Cnidium monnier group and Raloxifene group were significantly higher than that in OVX control group(P<0.01).Conclusion Using Cnidium monnier Cusson can be an effective therapy for osteoporosis and demonstrated definite alterations in IGF-1 levels and Vitamin D metabolite.