Effect Of Glutamine On Change Of Intestinal Microecology In Rats Exposed To Acute Plateau Hypoxia

Objective To observe cytokine and microecology (dysbacteriosis and bacterial translocation) changes of intestinal mucosa of glutamine (GLN) in rats exposed to acute high altitude. It can provide theoretical basis to prevent the imbalance of intestinal microflora in radical plateau hypoxia environment.Methods Wistar rats of 130 were randomly divided into A group(control group, altitude500 meters, Shanxi, xian), B group (altitude 3848 meters, Yuzhong, Maxian mountain), C group (altitude 3848 meters of glutamine intervention, Yuzhong, Maxian mountain), D group (altitude 4767 meters, plateau experiment center in Kekexili) and E group (altitude 4767 meters, plateau experiment center of glutamine intervention in Kekexili).A group have 10 rats. B and C groups were posed acute plateau hypoxia model to altitude 3848 meters, We drawn on the 2th,3th and 4th day, which kill 10 rats. D and E groups were posed acute plateau hypoxia model to altitude 4767 meters, We drawn on the 2th,3th and 4th day, which kill 10 rats. To detect the changes of feces smears oil microscopic observation, faeces pH value, dysbacteriosis and bacterial translocation and cytokine.Results 1 Intestinal flora imbalance rate in A group was 10%. B and D groups were discernly 53%and 86%, which were statistically significant than A group(P<0.05). C and E groups of glutamine intervention were discernly 46%and 70%, which were statistically significant than corresp plateau group(P<0.05).2 Faeces pH value in A group was 10%. B and D groups were discernly 53%and 80%, which were statistically significant than A group(P<0.05). C and E groups of glutamine intervention were discernly 40%and 67%, which were statistically significant than corresp plateau group(P<0.05).3 Intestinal bacterial translocation rate in B group was not significant than A group(P>0.05). Intestinal bacterial translocation rate in D group was significant than A group(P<0.05). Intestinal bacterial translocation rates in C and E groups of glutamine intervention were significant than corresp plateau group(P<0.05).4 The TNF-a and IL-6 contents of intestinal tissue in B and D groups were significantly higher than A group. The TNF-a and IL-6 contents of intestinal tissue in C group was lower than B group, the difference has statistincal significance(P<0.05). The TNF-a and IL-6 contents of intestinal tissue in E group was lower than D group, the difference has statistincal significance(P<0.05).Conclusion Exposure to radical plateau hypoxia can influence the imbalance of intestinal flora, displaced bacterial translocation, increased cytokine expression and mucosa structure of small intestine, finally results in damage of intestinal mucosal barrier. These changes were more evident with the elevation and time. Glutamine can have the roles of protecting the mucosal barrier and regulating the intestinal flora of disbalance.