The Significance Of DNA Damage In Peripheral Blood Lymphocytes Of Patients With Chronic Obstructive Pulmonary Disease

Objective To explore the levels of DNA damage in peripheral blood lymphocytes and its correlation analysis with the Chronic Obstructive Pulmonary Disease (COPD).Methods Thirty patients with COPD and twenty-four healthy controls (control group) which all diagnosed by pulmonary function testing were chosen from The First Hospital of Lanzhou University,The levels of genetic damage in peripheral blood lymphocytes from 30 patients with COPD and 24 healthy controls was evaluated by cytokinesis-blocked micronucleus(CBMN) methods, the frequencies of micronuclei(MN), nucleoplasmic bridges (NPBs),nuclear buds(NBUDs) were used to investigate the DNA damage.Results Compared with control subjects, mean frequencies of micronuclei, nucleoplasmic bridges and nuclear buds for patients with COPD showed an statistically significant (P<0.01). The frequencies of micronuclei, nucleoplasmic bridges and nuclear buds were compared for patients in groups according to COPD severity as defined by GOLD, the moderate, severe, very severe showed differ significantly (P<0.05). In patients with COPD, there was negative correlation between MN, NPBs and degree of airflow obstruction by FEV1% predicted (rs=-0.0401,P=0.028; rs=-0.549,P=0.002). The level of showed a strongly negative correlation with DNA damage Conclusions The result of CBMN in our study indicate an increased level of DNA damage in patients with COPD, The levels of DNA damage may be have a correlation with COPD severity.