Association Study Of IL-27 Gene Polymorphism With Asthma In Han Nationality Of Northeast Correlation

Objective:To detect the relationship of IL-27p28 gene polymorphisms in promoter region g.-964A>G and the second exon g.2905T>G with the susceptibility to asthma in northeastern China population. Methods:111 healthy controls were recruited from the general population who took a comprehensive medical examination at northeast region, and asthma patients were recruited from the northeast. Genomic DNA were extracted from the whole blood using the methods of small dosage with DNA extraction reagents. IL27p28 gene was amplified from genomic DNA by polymerase chain reaction method. Then, the fragments containing g.-964A>G, and g.2905T>G were directly sequenced, and the genotypes of these two sites were recorded. The cases and controls were compared by case-control association analysis. Fisher's Exact Test was used to detect deviation from Hardy-Weinberg equilibrium. Genotype and allele frequencies were compared between healthy controls and asthma patients by chi-square test. Results:All genetic variants were in Hardy-Weinberg equilibrium. A/A, A/G, G/G genotypes were detected in g.-964 locus and the genotype frequencies in asthma patients showed: A/A, A/G, G/G was 0.524,0.238,0.238 respectively, in healthy control were 0.486> 0.423,0.091 respectively. Allele frequencies showed:A was 0.643, G was 0.357 in asthma patients,0.698 and 0.302 in healthy control, p value was 0.082 and 0.477 by chi square examination.T/T,T/G,G/G genotypes were detected in 2905T>G locus in healthy controls, but in asthma patients only T/T,T/G genotypes. The genotype frequencies in asthma patients showed:T/T,T/G,G/G was 0.833,0.167,0 respectively, in healthy control were 0.838,0.152,0.010 respectively. Allele frequencies showed:T was 0.917, G was 0.083 in asthma patients,0.914 and 0.086 in healthy control. p value was 0.880 and 0.477 by chi square examination. Conclusion:IL-27p28 gene polymorphisms-964A>G,2905T>A might not be associated with asthma susceptibility in Northeastern Chinese Han population, but does not rule out the possibility that other genes within the polymorphic sites associated with asthma.