Study On Anti-Hepatic Injury Activity Of Geum Aleppicum Jacq

Objective (1) To investigate the free radical scavenging activity of extracts of 60 medicinal plants in the Changbai Mountain areas, and to measure their content of total phenolic compounds, flavonoids and flavonols. (2) To observe the protective effect of Geum aleppicum Jacq. aqueous extract against carbon tetrachloride induced acute liver injury in mice. Method (1) Extracts of 60 medicinal plants were investigated for their free radical scavenging activity activity using DPPH assay experiment. (2) The content of total phenolic compounds, flavonoids and flavonols was measure in plant extracts using colorimetric method. (3) 60 male mice were randomly divided into normal control group, model control group, high dosage group, middle dosage group, low dosage group and positive control group. The mice in the above groups were fed for 6 days. The drug groups were pretreated intragastrically with 2.0,1.0,0.5 g/kg extract. Positive control group was pretreated intragastrically with 0.1 g/kg silybin. Control mice were given the same volume physiological saline. On the 5th day of feeding, the mice except normal control group were given intraperitoneally 2.0 mL/kg 0.1%carbon tetrachloride. Twenty four hours after the intoxication, the serum ALT, SOD activities and MDA content were measured in each group above. Result (1) Geum aleppicum Jacq., Terminalia chebula Retz. aqueous extract exhibited very strong DPPH radical scavenging activity and had a high content of total phenolic compounds. (2) Geum aleppicum Jacq. aqueous extract significantly decreased carbon tetrachloride induced acute hepatic injury with increasing the activity of ALT and the content of MDA, increased the activities of SOD in serum. Conclusion It is revealed that Geum aleppicum Jacq.aqueous extract could prevent the CCl4 from inducing acute liver injury in mice through its antioxidation.