Parallel Test And Clinical Signification Of Serum Tumor Necrosis Factor α (TNF-α) And Interferon γ (IFN-γ) In Patients With Hepatitis B.

Aim:To study the relationship and clinical significance between serum tunor necrosis factor a(TNF-a)and interferonγ(IFN-γ) by testing the levels of serum TNF-αand IFN-γin patients with hepatitis B cirrhosis, chronic hepatitis B, hepatitis B virus carriers and health adults. Besides, to investigate the role of these two cytokines in the pathogenesis of hepatitis B.Methods:The subjects are divided into six groups:1) hepatitis B cirrhosis Childpugh C group (n=30); 2) hepatitis B cirrhosis Childpugh B group (n= 30); 3) hepatitis B cirrhosis Childpugh A group (n= 30); 4) chronic hepatitis B group (n= 30); 5) hepatitis B virus carriers group (n= 30); 6) health adults control group (n= 20). The levels of TNF-a and IFN-Y were detected by ELISA method. The levels of serum ALT, AST, TBIL, TBA and other biochemical parameters were examined by automatic biochemical analyzer synchronously. The levels of serum HBV-DNA was detected by fluorescence PCR and presented as Ig (HBV-DNA). Pearson correlation analysis was applied to study the relationship between serum TNF-α,IFN-γlevel and the severity of liver injury.Results:1) The levels of serum TNF-αand IFN-γin 150 cases of patients with hepatitis B were significantly lower than the control group(TNF-α:F=35.813, P < 0.01; IFN-γ:F=12.369, P< 0.01). However, there was no statistical significance difference between groups with hepatitis B. Pearson correlation analysis revealed: 2)the levels of serum TNF-a negatively correlated with the levels of serum AST, ALT, TBIL, TBA, PT and HBV-DNA(P< 0.05); 3) the levels of serum IFN-Y and serum levels of AST, ALT, TBIL, PT were no correlation(P>0.05),but negatively correlated with TBA and HBVDNA(P<0.05);4)In the healthy control group, the levels of serum TNF-αand IFN-γwere higher than hepatitis B group clearly.Conclusion:1) In hepatitis B patients, the levels of serum TNF-αand IFN-γwere significantly reduced.2) The higher are the levels of serum AST, ALT, TBIL, TBA, PT and HBV-DNA,the lower are the levels of serum TNF-α.3) At the same time, the higher are the levels of serum TBA and HBV-DNA load, the lower are the levels of serum IFN-γ.4) The role which TNF-αand IFN-γused in the pathogenesis of hepatitis B may be the same.