| The Gastric cancer is the most common malignant tumor of alimentary tract and the mortality rate accounts the first in total carcinomas. Radical surgical resection, of which the relapse and metastasis are common, is the main therapeutic method for gastric cancer by now and a lot of patient die from relapse and metastasis. Invasion and metastasis are important biological feature behavior of malignant tumors. The tumor cell adhesion and transfer of movement of its invasion and metastasis have a major impact. The transfer of the cells derived from cell adhesion and regulatory factors both within and outside the cell adhesion and cytoskeleton regulation. However, with the development of the cell biology and molecular biology of tumor invasion and metastasis, we have a more in-depth understanding of the process, It can be basically summarized as:cancer invasion and metastasis in cells prior to adhesion to lift its own; cancer stick is attached to substrate; cancer cells release matrix degrading proteolytic enzymes (ECM); cancer and other transitional cell. This in-depth research can enhance our understanding of gastric cancer invasion and transfer of control mechanism for the understanding and awareness, seeking to accurately determine the potential of invasion and metastasis of gastric cancer molecular markers for early diagnosis of gastric cancer metastasis, prognosis, targeted therapy for drugs provide a theoretical basis. Human Fascin protein in the cytoplasm of the cell membrane processes and the composition of such micro-tow work, whose structures play a role in cell adhesion and movement, moreover internal and external signals for cell regulation, cell adhesion protein E-cadherin and the cytoskeleton protein Fascin have play a significant in gastric cancer by the cell adhesion and metastasis. D-limonene (D-limonene, D-L) is a natural monoterpenoids in the essential oils of orange peel which contents supior to 90%,it has found that anti-cancer activity of D-L, for the prevention and treatment of chemical carcinogen-induced colon cancer, breast cancer, gastric cancer, lung cancer, skin cancer, liver cancer, pancreatic cancer and so made a considerable impact, however, it's low toxicity, characteristics such as broad-spectrum and makes the food has important research value. This research of Fascin protein and E-cadherin protein expression in gastric cancer cell, have to observe the D-limonene on human gastric cancer cell (SGC-7901), as well as clinical significance,so the expression of Fascin protein and cell adhesion protein E-cadherin impact studies under the D-limonene in the anti-tumor adhesion and metastasis,which provide a new basis in the future.Objective:To research the D-limonene effects on gastric cancer cells (SGC-7901) adhesion protein E-cadherin and the cytoskeleton protein Fascin, and the relationship between the two gastric Fascin and E-cadherin protein expression, so as to discuss the relationship between the two proteins and its biological behavior of gastric cancer impact.Methods:1.make use of SP immunohistochemical assay Fascin and E-cadherin protein in 100 cases of gastric cancer tissue. Statistics by spssl8.0 statistical software.2 make use of Immunocytochemical method to detect the role of the concentration of D-limonene of gastric cancer cells (SGC-7901) after the E-cadherin and gastric cancer cells in their expression of Fascin3 make use of the Quantitative Western blot detection of the role of different concentrations of D-limonene of gastric cancer cell E-cadherin and the expression of Fascin.Results:1.Fascin and E-cadherin protein in gastric cancer tissues were correlated with tumor differentiation, depth of invasion, lymph node metastasis, clinical TNM staging closely related (P<0.05). at the same time the Fascin and the E-cadherin protein expression was negatively correlated (r=-0.74, P＜0.05). 2 The role of D-limonene, the gastric cancer cells (SGC-7901) Fascin protein with D-limonene concentration, cytoplasmic staining decreased gradually. Western blot showed:Fascin protein content increased with drug concentration, the more obvious reduction. That D-limonene by reducing the expression of Fascin protein to reduce the invasiveness of gastric cancer cells.3 The role of D-limonene, the gastric cancer cells (SGC-7901) E-cadherin protein with D-limonene concentration, membrane staining increased gradually. Western blot showed:E-cadherin protein increasing drug concentration, increased more significantly. That D-limonene by increasing E-cadherin protein to enhance the adhesion of gastric cancer cells.Conclusions:Fascin over-expression and low expression of E-cadherin in early gastric cancer are important factors in metastasis, it detect gastric cancer metastasis as an important indicator of potential, but also for evalua-ting the biological behavior of tumors, the prognosis of patients estimated to provide a reference value, D-limonene through on gastric cancer cells re-duces the expression of Fascin protein to suppress the invasi-ve ability of tumor cells, on the E-cadherin protein expression increased to enhance cell adhesion, thereby co-inhibition of gastric cancer cell invasion and metastas-is。...
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