Expression And Clinical Significance Of Akt2 And Beclin 1 In Primary Hepatic Carcinoma

Objective: To detect Akt2 and Beclin 1 expression in primary hepatic carcinoma (PHC), cirrhotic liver and normal liver tissues, and study their correlations to the clinic pathological features. To explore the effect of Akt2 and Beclin 1 expression in the occurrence,development and metastasis of primary hepatic carcinoma and their interrelation.Method: The expression of Akt2 and Beclin 1 in 76 cases of primary hepatic carcinoma tissue,54 cases of cirrhotic liver tissue and 20 cases of normal liver tissues were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Elivision immunohistochemistry. The condition of the clinical pathological parameters and their interrelation were analyzed using statistical method.Result:1. Akt2 was mainly expressed in primary hepatic carcinoma and cirrhotic liver tissues, there was low expression in normal liver tissues. The Akt2 mRNA expression were 0.90±0.14,0.19±0.05,0.02±0.02 in PHC tissues,cirrhotic liver tissues and normal liver tissues, the difference was statistically significant(F=3.069,P<0.05); The Akt2 protein expression were 90.79%,25.92%, 5% in PHC tissues,cirrhotic liver tissues and normal liver tissues, the difference was statistically significant(χ2=82.881,P<0.05). The expression of Akt2 showed no obvious correlations with the age, gender, Edmondson-steiner grade, tumor size, HBSAg and AFP (P>0.05),but was related to portal vein tumor thrombi and biliary tumor thrombi and the degree of hepatic cirrhosis (P<0.05).2. Beclin 1 was also mainly expressed in primary hepatic carcinoma and cirrhotic liver tissues and there was low expression in normal liver tissues. The Beclin 1 mRNA expression were 0.78±0.10,0.39±0.04,0.04±0.02 in PHC tissues,cirrhotic liver tissues and normal liver tissues, the difference was statistically significant(F=5.78,P<0.05); The Akt2 protein expression were 84.21%,37.04%,10% in PHC tissues,cirrhotic liver tissues and normal liver tissues, the difference was statistically significant(χ2=49.85, P<0.05). The expression of Beclin 1 showed no obvious correlations with the age, gender, Edmondson-steiner grade, tumor size, HBSAg, AFP and the degree of hepatic cirrhosis (P>0.05),but was related to portal vein tumor thrombi and biliary tumor thrombi (P<0.05).3. The expression rate of Akt2 was significantly correlated with that of Beclin 1 in primary hepatic carcinoma(r=0.947,P=0.04).Conclusion: There were higher expression of Akt2 and Beclin 1 in primary hepatic carcinoma. Akt2 and Beclin 1 interact one another and participate in the occurrence,development and metastasis of PHC. Combined detection of Akt2 and Beclin 1 has important value for prediction of occurrence and metastasis. They are both potential target gene for the therapy of PHC.