| Objective:According to the pathogenesis of Cerebral infarction's disease in both Chinese and western medicine as well as the research progress on Rhizoma Corydalis decumbentis, using Classical medicine venoruton as a control drug. Observing each group's change of the pathomorphology of brain tissue by HE staining, and determining in serum high-sensitive C-reaction protein (hs-CRP) concentrations and brain tissue of brain-derived neurotrophic factor (BDNF) levels. To observe Rhizoma Corydalis decumbentis's brain neuroprotective effect on cerebral infarction's model rats,and explore effective mechanisms for its treatment of cerebral infarction., to provide the experimental basis for reliable clinical medication.Methods:1.60 Healthy male wista rats were weighed 200～250g, and divided into two groups at random normal group(n=8), sham operation group(n=8), and made module(44).2. The other 44 rats were made for the rat model of cerebral infarction by the method of middle cerebral artery occlusion (MCAO). Except the 2 death rats and 6 unsuccessful rat models, randomly chose 32 rats from the 36 successful rat models and randomly divided them into four groups:model group, high doses group, low doses group and Venoruton control group.3. Each group took fasting from the previous day of operation, fed drugs from the next day of operation, and collected indications on the seventh day of being given drugs.4. The rats in normal group, sham operation group and model group were given gavage with normal saline 6ml/Kg/d for 7 days; the rats in high doses group were given gavage with corydalis decumbens 9.6g/Kg/d for 7days (the dose was four times as much as that in low doses group); the rats in low doses group were given gavage with corydalis decumbens 2.4g/Kg/d for 7days (the dose was 20 times as much as the daily normal dose as per a adult's weight in kg.); the rats in Venoruton control group were given gavage with Venoruton 144mg/kg/d for 7days (the dose was 20 times as much as the daily normal dose as per the adult's weight in kg).5. Used spectrophotometry to detect the activities in serum high-sensitive C-reaction protein (hs-CRP) concentrations.6. Used immunohistochemical detection to analyze the protein expression of brain-derived neurotrophic factor (BDNF) levels.7. Observing each group's change of the pathomorphology of brain tissue by HE stainingResults:1. Detecting Serum high-sensitivity C-reactive protein (hs-CRP) concentration: the high dose group of Rhizoma Corydalis decumbentis, low dose group of Rhizoma Corydalis decumbentis,and the group of Venoruton's hs-CRP concentrations were lower than model group (P<0.01).And Compareing with low dose group, the high dose group and Venoruton group decreased more significantly (P<0.001),the high dose group and Venoruton group was not statistically significant (P>0.05)2. Detection of BDNF levels:the high dose group of Rhizoma Corydalis decumbentis, low dose group of Rhizoma Corydalis decumbentis,and the group of Venoruton's BDNF levels were higher than model group (P<0.01).And Compareing with low dose group, the high dose group and Venoruton group increased more significantly (P<0.001),the high dose group and Venoruton group was not statistically significant (P>0.05).3. The effects of corydalis decumbens on the pathomorphology of brain tissue in the rat model of cerebral infarction. In sham operation group and normal group, the nerve cells of the rat's cerebrum and cerebellum worked normally with clear nucleolus, karyotheca and cellmembrane; the neuroglia cell was in normal condition with clear nucleolus and karyotheca; the sarcoplasma was translucent or pale. The essential of the rat's brain in model group became degeneration or even necrosis, which showed pycnosis, cytoplasm's deep dyeing for blue, unclear nucleus structure, shrinkage of the cell, neurites with acute angle, some nucleolus and karyotheca disappeard and the nucleus was dissolving; the neuroglia cell was tumid. The pathological changes of brain's nerve cell and neuroglia cell in high doses group, low doses group and Venoruton control group decreased evidently with fewer necrotic cells and no disappear of never cell's nucleus. Among them, the effects in high doses group were the most obvious while the effects in low doses group and Venoruton control group were similar to each other.Conclusions:1. The operation is simple but with high success rate to prepare the rat model of cerebral infarction by middle cerebral artery occlusion (MCAO) which is the most wide and successful method of focal cerebral infarction in rats at present.2. Traditional Chinese medicine Rhizoma Corydalis decumbentis significantly inhibited the inflammatory hs-CRP increased, suggesting that Rhizoma Corydalis decumbentis may have no role in stroke prevention and treatment.3. Rhizoma Corydalis decumbentis can improve cerebral infarction in rat brain BDNF levels, promote their generation, suggest that promote brain cell regeneration and the prevention of nerve cell degeneration Rhizoma Corydalis decumbentis the mechanisms of effective treatment of cerebral infarction.4. Traditional Chinese medicine Rhizoma Corydalis decumbentis can improve the pathomorphology of brain tissue., prompting that Rhizoma Corydalis decumbentis has a definite therapeutic effect on cerebral infarction.5. Traditional Chinese medicine Rhizoma Corydalis decumbentis and Classical medicine venoruton can treat cerebral infarction and were more equivalent role in their treatment with the drug concentration increased with the increase.
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