Experimental Study On The Effect Of Corydalis Decumbentis On Circulating Endothelial Cells And Myelin Basic Protein In Cerebral Infarction Rats

Objective:According to the in-depth study of the pathogenesis of traditional Chinese and western medicine in cerebral infarction and the research development to corydalis decumbens, the experiment takes Venoruton, an effective medicine in treating cerebral infarction, as a control medicine, observe the effects of corydalis decumbens on circulating endothelial cells(CEC),myelin basic protein(MBP) and the pathomorphology of brain tissue in the rat model of cerebral infarction, and then investigate the possibility of corydalis decumbens in treating cerebral infarction so as to guide clinical medication.Methods:1. Prepared 60 Wistar male rats that were healthy and weighed 225±25g, randomly chose 16 rats and divided into two groups at random:the normal group and the sham operation group in which there were 8 rats. The other 44 rats were made for the rat model of cerebral infarction by the method of middle cerebral artery occlusion (MCAO). Except the 2 death rats and 6 unsuccessful rat models, in order to maintain the same group samples, reduce system error, removing 2 the front and 2 the end in the 36 successful rat models, the rest of the 32 only randomly were divided them into four groups:model group, high doses group, low doses group and Venoruton control group in which there were 8 rats.2.Each group took fasting from the previous day of operation, fed drugs from the next day of operation, and collected indications on the seventh day of being given drugs.3.The rats in normal group, sham operation group and model group were given gavage with normal saline 6ml/Kg/d for 7 days; the rats in high doses group were given gavage with corydalis decumbens 9.6g/Kg/d for 7days (the dose was four times as much as that in low doses group); the rats in low doses group were given gavage with corydalis decumbens 2.4g/Kg/d for 7days (the dose was 20 times as much as the daily normal dose as per a adult's weight in kg.); the rats in Venoruton control group were given gavage with Venoruton 144mg/kg/d for 7days (the dose was 20 times as much as the daily normal dose as per the adult's weight in kg).4. After the operations to each group, took blood collection from abdominal aorta by Percoll density gradient separation method to identify the number of CEC in rat's blood plasma of each group, used antibody_sandwich ELISA method to identify the MBP in blood serum, and then observed the changes in the pathomorphology of brain tissue after dyeing the brain with HE.Results:1.Percoll density gradient separation method to identify the number of CECThe compare for the number of CEC in model group with that in normal group and sham operation group had significant statistical significance (P<0.001); the number of CEC in high doses group and low doses group of corydalis decumbens and Venoruton group is lower than that in model group, which had obvious statistical significance (P<0.01). Besides, the decrease of the number of CEC in high doses group was quite obvious compared with that in Venoruton group and low doses group(P<0.05). Neither the compare between low doses group and Venoruton group nor the compare between normal group and sham operation group had statistical significance (P>0.05).2.ELISA method to identify the MBP density of blood serumThe compare for the MBP density in model group with that in normal group and sham operation group has significant statistical significance (P<0.01); the MBP density in high doses group and low doses group of corydalis decumbens and Venoruton group was lower than that in model group, which had statistical significance (P<0.05). Besides, the decrease of the MBP density in high doses group was quite obvious compared with that in Venoruton group and low doses group (P<0.05). Neither the compare between low doses group and Venoruton group nor the compare between normal group and sham operation group had statistical significance (P>0.05)3.The effects of corydalis decumbens on the pathomorphology of brain tissue in the rat model of cerebral infarctionIn sham operation group and normal group, the nerve cells of the rat's cerebrum and cerebellum worked normally with clear nucleolus, karyotheca and cellmembrane; the neuroglia cell was in normal condition with clear nucleolus and karyotheca; the sarcoplasma was translucent or pale. The essential of the rat's brain in model group became degeneration or even necrosis, which showed pycnosis, cytoplasm's deep dyeing for blue, unclear nucleus structure, shrinkage of the cell, neurites with acute angle, some nucleolus and karyotheca disappeard and the nucleus was dissolving; the neuroglia cell was tumid. The pathological changes of brain's nerve cell and neuroglia cell in high doses group, low doses group and Venoruton control group decreased evidently with fewer necrotic cells and no disappear of never cell's nucleus. Among them, the effects in high doses group were the most obvious while the effects in low doses group and Venoruton control group were similar to each other.Conclusion:1.The operation can be successfully replicated the rat model of cerebral infarction by middle cerebral artery occlusion (MCAO),2. The mechanism of Corydalis decumbens treating cerebral infarction relates to reduce vascular endothelial cells's apoptosis.3. The protective effect of Corydalis decumbens treating cerebral infarction can be proved by brain cells degeneration and necrosis of tissue.4 The effect of Corydalis decumbens treating cerebral infarction is that high dose is better than low dose.